- 询价
- Signalway Antibody
- 21581
- 2025年09月03日
- WB
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around aa.437-441(V-D-S-V-E) derived from Human alpha-Tubulin.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
Uniprot：P68363
- 抗体英文名：
a-Tubulin Antibody
- 规格：
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Predicted MW: 52kd
Western blotting: 1:500~1:1000
图片:
Western blot analysis of extracts from Hela and HT29 cells using alpha-Tubulin Antibody #21581.
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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
. Scan the gels or photographically analyze them. Determine the molecular weights of the proteins (tubulin and MAP's).
Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
I. Useful Values 1 mg/ml tubulin = 10 µM (assuming MW of ab-tubulin heterodimer is 100,000; in reality it is ~110,000 but almost all tubulin labs use this convenient conversion relationship) 1 µm of a microtubule has ~1600
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