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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant full length protein (352aa) of mouse POU5F1
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Mouse
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Mouse
- 应用范围:
WB ICC FC
- 靶点:
Uniprot:Q01860
- 抗体英文名:
POU5F1 Monoclonal Antibody
- 规格:
50ul/100ul
配方:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
应用详情:
Predicted MW: 45kd
Western blotting: 1:1000
图片:

1:1000 dilution of this antibody detected F9 on 40ug of cell lysates
,

1:100 dilution from a previous lot detected POU5F1 in Hela
,

F9 cells stained with POU5F1 (red, 1:100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody-Based Immunoassays
An immunoassay may be defined as an assay that employs an immunological reagent, usually an antibody, to confer specificity for the ligand being measured. As a corollary to this, the discovery, and subsequent development, of monoclonal
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