- 询价
- Signalway Antibody
- 42389
- 2025年09月22日
- WB
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Actin-related protein 2/3 complex subunit 3 protein
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
Uniprot：O15145
- 抗体英文名：
Actin-related protein 2/3 complex subunit 3 Polyclonal Antibody
- 规格：
50ul/100ul
计算分子量:20kd
配方:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
应用详情:Western blotting: 1:500 - 1:1000
图片:
Actin-related protein 2/3 complex subunit 3Antibody at 2ug/ml + 293T whole cell lysate at 20ug SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 20 kDa Observed band size : 75 kDa
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文献和实验Combine actin + GST-fusion protein and incubate for 1 hr. x 4¡C. Add 20ul 50% glutathione agarose equilibrated into binding buffer. Incubate 15 min x 4¡C with frequent mixing. Wash beads 4 times with binding buffer, spin down 4-8000 rpm
by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
buffer. Combine actin + GST-fusion protein and incubate for 1 hr. x 4¡C. Add 20ul 50% glutathione agarose equilibrated into binding buffer. Incubate 15 min x 4¡C with frequent mixing. Wash beads 4 times with binding
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