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文献和实验DNA提取试剂盒; EcoRI酶,MseI酶,T4连接酶试剂盒; Taq酶,dNTP, PCR reaction buffer; AFLP引物; 琼脂糖电泳试剂:琼脂糖,无毒GeneFinder核酸染料替代传统EB染料; 超纯水(18.2MΩ·cm) 2.其他实验需要物品 微量移液枪(一套)及相应尺寸Tip头,PCR管,冰浴等。 四
AFLP: not only for fingerprinting, but for positional cloning
.] [Note 3: The AFLP procedure reported in NAR by Vos et al. (1995) uses a preamplification with both EcoRI and MseI primers having 1-bp, 3'-extensions, followed by AFLP-PCR with primers having 3-bp, 3'-extensions, whereas our preamplification uses
). This thermocycler accepts 96-well microtiter plates, which is a more efficient way to amplify the multiple samples required for AFLP.] [Note 3: The AFLP procedure reported in NAR by Vos et al. (1995) uses a preamplification with both EcoRI and MseI primers
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