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MS培养基(含维生素B5), MS Medium/Muras

hige & Skoog medium(with B5 vitamins)
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  • 2025年08月24日
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    • 英文名

      MS Medium/Murashige & Skoog medium(with B5 vitamins)

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      现货

    MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White’s medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana cells significantly, however a concentration of 80 mM and higher was clearly disadvantageous to the cells. The increase of all other elements, especially the macro -elements, also stimulated the growth of the calli. Due to the high concentration of minerals, MS medium is a very rich and -saline -medium and may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors. Sometimes the original MS vitamines are replaced by the vitamins of Linsmaier and Gamborg B5 medium regarding the higher concentration of Thiamine in relation to the requirement of this vitamin by plants.
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    图标文献和实验
    相关实验
    • 常用试剂和培养基

      succinate and add 10 ml glucose for storage medium. E. coli minimal 200 ml 1000 ml 10X M9 .......... 20 ml 100 ml MgSO4 (0.1 M) ... 2 ml 10 ml CaCl2 (0.5 M) ... 0.2 ml 1 ml 1000X vitamins .. 0.2 ml 1 ml Glucose (40%) ... 2 ml

    • 常用试剂和培养基

      succinate and add 10 ml glucose for storage medium. E. coli minimal 200 ml 1000 ml 10X M9 .......... 20 ml 100 ml MgSO4 (0.1 M) ... 2 ml 10 ml CaCl2 (0.5 M) ... 0.2 ml 1 ml 1000X vitamins .. 0.2 ml 1 ml Glucose (40%) ... 2 ml

    • In Vitro Propagation of Jojoba

      is limited by the size of the plants and time of the year. Micropropagation is highly recommended strategy for obtaining jojoba elite clones. For culture initiation, single-node explants are cultivated on Murashige and Skoog medium (MS) supplemented

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