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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
低温
- 英文名:
Lenti-X™ 293T Cell Line
- 库存:
大量
- 供应商:
上海善然生物科技有限公司
- 规格:
1 ml
At-A-Glance Documents Images & Data Resources
Features
293T clone selected for high titer lentivirus production
Get more lentivirus produced per transfection
6X more virus than 293FT cells, 30X more virus than HEK 293 (see images and data tab)
Obtain up to 109 IFU from one 10 cm plate when combined with Clontech’s Lenti-X HTX Packaging System
Products
Cat. # Product ContentsSize632180Lenti-X™ 293T Cell Line 1 mL
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| 温馨提示:建议细胞产品接收后立即进行复苏培养,如若不能立即复苏培养则需要保存在液氮中或者-150℃冰箱中,并尽早进行复苏培养。对于HEK 293细胞,建议采用胶原蛋白包被培养皿辅助细胞贴壁。 |
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- 询问日期
文献和实验293T: Add 1 ml 0.05% Trypsin & EDTA to a full 100 mm plate (~1.2-1.4 x 107 cells), Add 5 ml fresh medium to terminate reaction, Count cells and adjust to a concentration of 2x 106 cells / ml. 5. Aliquot 1 ml cells to a well of 6-well plate (2x
关于293/293T细胞1、种属都为人,不是鼠。2、来源:293细胞是转染腺病毒E1A基因的人肾上皮细胞系,293T细胞由293细胞派生,同时表达SV40大T抗原,含有SV40复制起始点与启动子区的质粒可以复制。用Ca3(PO4)2转染效率可高达50%。蛋白表达水平高,转染后2-3天用碱性磷酸酶分析可较容易地检测到表达的蛋白。瞬时转染293T细胞是过表达蛋白并获得细胞内及细胞外(分泌的或膜)蛋白的便捷方式。3、形态为上皮细胞,能致瘤。4、染色体核型:亚三倍体人细胞系。染色体众数为64,30
Transient transfection into 293T cells
Purpose Transient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1
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