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- 详细信息
- 技术资料
- 保存条件：
负20度
- 保质期：
2年
- 英文名：
pACYC177
- 库存：
100
- 供应商：
kelei-bio
- 规格：
20μl
基本信息
复制子: p15A ori
质粒分类: 大肠杆菌载体；克隆质粒
质粒大小: 3941bp
原核抗性: 氨苄青霉素Amp；卡那霉素Kan
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
5'测序引物: 根据序列设计
3'测序引物: 根据序列设计
备注: 丰富的酶切位点
质粒简介
pACYC177 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC177 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with chloramphenicol or spectinomycin. Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence.
复制子: p15A ori
质粒分类: 大肠杆菌载体；克隆质粒
质粒大小: 3941bp
原核抗性: 氨苄青霉素Amp；卡那霉素Kan
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
5'测序引物: 根据序列设计
3'测序引物: 根据序列设计
备注: 丰富的酶切位点
质粒简介
pACYC177 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC177 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with chloramphenicol or spectinomycin. Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence.
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pACYC177质粒
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