- 询价
- ATCC
- TIB-71
- 美国
- 2025年11月17日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
ATCC
- 细胞类型：
单核细胞/巨噬细胞
- ATCC Number：
TIB-71
- 组织来源：
小鼠（小家鼠）
- 物种来源：
Mus musculus, mouse
- 器官来源：
腹水
- 运输方式：
干冰
- 生长状态：
贴壁
RAW 264.7; Macrophage; Mouse (Mus musculus)
General
Specific applications
This cell line is a suitable transfection host.
The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets.
该细胞系是合适的转染宿主。细胞将呈中性红色，吞噬乳胶珠和酵母聚糖。它们能够对绵羊红细胞和肿瘤细胞靶点进行抗体依赖性裂解。LPS或PPD治疗2天刺激红细胞裂解，但不刺激肿瘤细胞靶标。
Characteristics
Growth properties
Adherent
Derivation
This line was established from a tumor induced by Abelson murine leukemia virus.
Age
adult
Gender
Male
Strain
BALB/c
Immortalization method
Abelson murine leukemia virus transformed
Antigen expression
H-2d
Genes expressed
lysozyme; H-2d; ectromelia virus (mousepox) negative.
Expression markers
Complement (C3)
Comments
This cell line is easy to propagate, high efficiency for DNA transfection, sensitivity to RNA interference, and supports replication of murine viruses. This cell line is negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2). When this line was established, it was described as not secreting detectable virus particles and negative using the XC plaque formation assay. Based on a published study by Dr. Janet W. Hartley in 2008, this line was demonstrated to express ecotropic and polytropic MuLV, and is positive using the XC plaque assay for virus replication.
该细胞系易于繁殖，DNA转染效率高，对RNA干扰敏感，并支持小鼠病毒的复制。该细胞系对表面免疫球蛋白（sIg-）、Ia（Ia-）和Thy-1.2（Thy-1.2）呈阴性。当建立该系时，它被描述为不分泌可检测的病毒颗粒，并且使用XC噬斑形成试验呈阴性。根据Janet W.Hartley博士2008年发表的一项研究，该系被证明表达嗜生态和多嗜性MuLV，并且使用XC噬斑试验检测病毒复制呈阳性。
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- 作者
- 内容
- 询问日期
文献和实验Curated Citations
Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583
Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116
Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776
细胞学堂丨养不好RAW 264.7，是因为忽略了这几点，RAW264.7分化有哪些问题要注意
养过 RAW 264.7 细胞系的小伙伴，都容易为一个问题感到苦恼：它太容易分化了！到底怎样才能养好它呢？RAW264.7 细胞培养注意事项有哪些，今天我们就给大家分享一些心得。 RAW264.7 细胞培养之基础篇 RAW264.7 细胞培养的第一步，是要对它的基础培养条件了如指掌，比如生长特性、细胞形态、所需的培养基，甚至培养环境、传代比例等等，做到知己知彼，才能百战百胜。 ▲产品详情 细胞系：RAW 264.7
我养的是RAW264.7细胞株，前面有战友都提到过这种细胞的消化传代，但是因为这种细胞最大的特点是贴壁特别强， 每次传代都很难消化下来， 刚开始使用胰酶消化，发现37度， 消化15min细胞也还是吹不起来，后来又用单纯的用细胞刮刀刮的办法，但是这样养了一段时间，发现细胞损伤非常大，贴壁性变差，生长缓慢，后来使用了现在这个方法，到现在细胞的生长状况一直很好!简述如下：1) 将培养瓶内旧的培养液弃掉， 然后用D-Hanks液洗两次，(一定要洗干净，以免影响胰酶的消化作用)2) 加入0.25%胰酶
RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 cells
the excessive bone loss that occurs in numerous skeletal disorders. The RAW 264.7 murine cell line has proven to be an important tool for in vitro studies of OC formation and function, having particular advantages over the use of OCs generated from primary bone
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