- 询价
- ATCC
- CRL-3216
- 美国
- 2026年04月09日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
国内现货
- 供应商：
ATCC
- ATCC Number：
CRL-3216
- 组织来源：
kidney; Embryo
- 物种来源：
Homo sapiens, human
- 细胞形态：
epithelial
- 运输方式：
干冰运输
- 年限：
fetus
- 生长状态：
贴壁
- 英文名：
293T; Embryonic Kidney Cells; Human (Homo sapiens)
| Organism | Homo sapiens, human |
| Tissue | kidney; Embryo |
| Product Format | frozen 1 mL |
| Morphology | epithelial |
| Culture Properties | adherent |
| Age | fetus |
| Applications | 293T is an epithelial-like cell that was isolated from the kidney of a patient. This cell line was deposited by Stanford University and can be used in vaccine development. |
| Shipping Information | Frozen 1 mL |
| Storage Conditions | Vapor phase of liquid nitrogen |
| Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| Biosafety Level | BSL 2 ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies. Cells contain Adenovirus ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen |
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- 作者
- 内容
- 询问日期
文献和实验DuBridge RB, et al. Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system. Mol. Cell Biol. 7: 379-387, 1987. PubMed: 3031469
Pear WS, et al. Production of high-titer helper-free retroviruses by transient transfection. Proc. Natl. Acad. Sci. USA. 90: 8392-8396, 1993. PubMed: 7690960
关于293/293T细胞1、种属都为人，不是鼠。2、来源：293细胞是转染腺病毒E1A基因的人肾上皮细胞系，293T细胞由293细胞派生，同时表达SV40大T抗原，含有SV40复制起始点与启动子区的质粒可以复制。用Ca3(PO4)2转染效率可高达50%。蛋白表达水平高，转染后2-3天用碱性磷酸酶分析可较容易地检测到表达的蛋白。瞬时转染293T细胞是过表达蛋白并获得细胞内及细胞外（分泌的或膜）蛋白的便捷方式。3、形态为上皮细胞，能致瘤。4、染色体核型：亚三倍体人细胞系。染色体众数为64，30
包装细胞293T细胞的培养一、293T细胞的冻存1. 随着传代的次数增加，293T细胞会出现生长状态下降，出现突变等。所以要在细胞购进时就进行大量冻存，以保证实验的稳定性和持续性。2. 在细胞对数生长期进行冻存，增加细胞复苏成活率。3. 倒去细胞上清液，加入D-Hank's液洗去残留的培养基。4. 加入0.25%的胰酶，消化10-20s后倒去。5. 镜下观察细胞变圆，细胞间间隙加大时，加入新鲜培养基吹打混匀。6. 细胞计数。7. 将细胞离心，1000rpm，2min。8.根据计数结果加入
相关专题 293细胞 关于293/293T细胞 1、种属都为人，不是鼠。 2、来源：293细胞是转染腺病毒E1A基因的人肾上皮细胞系，293T细胞由293细胞派生，同时表达SV40大T抗原 ，含有SV40复制起始点与启动子区的质粒可以复制。用Ca3(PO4)2转染效率可高达50%。蛋白表达水平高，转染后2-3天用碱性磷酸酶分析可较容易地检测到表达的蛋白。瞬时转染293T细胞是过表达蛋白并获得细胞内及细胞外(分泌
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