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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 库存:
预定周期6周
- 供应商:
上海冠东生物科技有限公司
- 抗体英文名:
FITC Mouse Anti-Human PAC-1
- 规格:
50 Tests
DESCRIPTION
The PAC-1 antibody is derived from the fusion of SP-2 mouse myeloma cells with splenic lymphocytes from BALB/c mice that were immunized with human platelets.
The PAC-1 recognizes an epitope on the glycoprotein IIb/IIIa (gpIIb/IIIa, αIIbβ3) complex of activated platelets at or near the platelet fibrinogen receptor.
FORMAT
- Format
- FITC
- Excitation Source
- Blue 488 nm
- Excitation Max
- 494 nm
- Emission Max
- 520 nm
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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- 作者
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- 询问日期
文献和实验当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
FITC荧光二抗?――EarthOx新型DyLight 488绿色荧光
FITC荧光二抗?――EarthOx新型DyLight 488绿色荧光 FITC(Fluorescein Isothiocyanate,异硫*酸荧光素)是一种绿色荧光团,在免疫学实验里,经常被用于荧光团标记到相应的检测分子,如抗体上。FITC的纯品为黄色或橙黄色结晶粉末,易溶于水和酒精溶剂。FITC分子量为389.4,最大吸收光波长为 490~495nm,最大发射光波长为520~530nm,呈现明亮的黄绿色荧光。FITC在冷暗干燥处可保存多年,是目前应用最广泛的荧光
Mammalian Artificial Chromosome Formation in Human Cells After Lipofection of a PAC Precursor
An artificial chromosome is a synthetic structure that carries three fundamental components for its long-term survival, replication, and segregation after cell division. These components are telomeres, one or more replication origins











