- ¥1000
- XYbscience
- 中国/美国
- XY1177
- 2025年07月14日
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- 详细信息
- 技术资料
- 保存条件：
常温
- 保质期：
三年
- 英文名：
pMT/BiP/V5-His A
- 库存：
60
- 供应商：
信裕生物
- 规格：
5ug质粒
基本信息
| 质粒类型: | 昆虫细胞表达载体 |
|---|---|
| 启动子: | MT |
| 载体大小: | 3642 bp (查看载体序列) |
| 载体标签: | V5 Epitope Tag, His Tag (6x) |
| 载体抗性: | Ampicillin (氨苄青霉素) |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| XY1177 | pMT/BiP/V5-His A | 5ug质粒 |
¥1000.00 |
质粒图谱
载体描述
The DES®-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. The pMT/BiP/V5-His vector offers the following additional features:
- Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
- C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
- C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.
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