| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCAMBIA2300 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | Lac |
| 载体大小: | 8743 bp |
| 5' 测序引物及序列: | M13 Reverse：CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward：CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 新霉素（Neomycin） |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pcambia2300
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS AF234315 8742 bp DNA circular SYN 24-APR-2000
DEFINITION Binary vector pCAMBIA-2300, complete sequence.
ACCESSION AF234315
VERSION AF234315.1 GI:7638145
KEYWORDS .
SOURCE Binary vector pCAMBIA-2300
ORGANISM Binary vector pCAMBIA-2300
other sequences; artificial sequences; vectors.
REFERENCE 1 (sites)
AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.
TITLE The small, versatile pPZP family of Agrobacterium binary vectors
for plant transformation
JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)
PUBMED 7919218
REFERENCE 2 (bases 1 to 8742)
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
TITLE A comprehensive set of modular vectors for advanced manipulations
and efficient transformation of plants
JOURNAL Unpublished
REMARK Full description of constructs
REFERENCE 3 (bases 1 to 8742)
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
TITLE Direct Submission
JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
GPO Box 3200, Canberra, ACT 2601, Australia
FEATURES Location/Qualifiers
source 1..8742
/organism="Binary vector pCAMBIA-2300"
/mol_type="other DNA"
/db_xref="taxon:118407"
misc_feature complement(980..1980)
/note="STA region from pVS1 plasmid"
rep_origin complement(2573..3573)
/note="pVS1-REP; replication origin from pVS1"
misc_feature complement(3983..4243)
/note="bom site from pBR322"
rep_origin complement(4383..4663)
/note="pBR322 origin of replication"
CDS complement(4954..5748)
/note="aadA (kanamycin resistance) gene amplified from
pIG121Hm"
/codon_start=1
/product="aminoglycoside phosphotransferase"
/protein_id="AAF65399.1"
/db_xref="GI:7638147"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 6173..6198
/note="left border repeat from C58 T-DNA"
misc_feature 6265..6468
/note="CaMV 3'UTR (polyA signal)"
CDS complement(6506..7303)
/note="nptII (kanamycin resistance) gene"
/codon_start=1
/product="neomycin phosphotransferase"
/protein_id="AAF65400.1"
/db_xref="GI:7638148"
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(7334..8114)
/note="CaMV35S2; CaMV 35S promoter, duplicated"
promoter 8262..8316
/note="PlacZ; lacZ promoter"
CDS 8344..8577
/codon_start=1
/product="LacZ alpha fragment"
/protein_id="AAF65398.1"
/db_xref="GI:7638146"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
misc_feature 8358..8414
/note="pUC18 MCS; polylinker"
misc_feature 8656..8681
/note="right border T-DNA repeat"
ORIGIN
1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
4921 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
4981 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
5041 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
5101 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
5161 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
5221 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
5281 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
5341 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
5401 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
5461 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
5521 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
5581 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
5641 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
5701 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
5761 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
5821 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
5881 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
5941 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
6001 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
6061 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
6121 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
6181 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
6241 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg
6301 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
6361 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg
6421 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc tagctagagg
6481 atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
6541 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
6601 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
6661 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
6721 gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
6781 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
6841 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
6901 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
6961 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
7021 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
7081 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc agggcaccgg
7141 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
7201 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
7261 cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat cgacagatct
7321 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
7381 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg
7441 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
7501 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
7561 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
7621 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
7681 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
7741 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa
7801 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
7861 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
7921 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
7981 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
8041 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
8101 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc
8161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
8221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
8281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
8341 gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc
8401 aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
8461 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
8521 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc
8581 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
8641 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta
8701 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tg
//
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载体应用举例
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文献和实验相关实验
(1300) P1 P2 pCAMBIA 2300 M13R(-48) M13F(-47) PCANTB5E S1/M13R S6 pCAT3-enhancer RVP3 此载体无反向引物 pcDNA3.0 CMV-F/T7 SP6/BGH pcDNA3.1 T7 BGH pcDNA4 T7/CMV-F BGH pcDNA6 T7/CMV-F(J21025在T7前面） BGH pcDNAII T7 SP6 pCE2.1 M13F M13R pCEP4 pCEP-F EBV-R
。可包装30～45kb长的DNA片段，多用于基因文库构建。 (四) 大片段DNA克隆载体 人类和哺乳动物的基因组很大，甚至许多单个基因也相当大（如：DMD gene 2300kb） ，克隆分析就需要更大的基因载体。如近年来构建的一些载体：BAC，PI，YAC等。 1、细菌人工染色体（bacterial artificial chromosome,BAC） 优点：能携带大片段DNA，约300kb。 在每个细胞中，一个载体分子能繁殖多个拷贝，高产DNA。 缺点：会出现插入片段在结构上
7-TerminatorpCAMBIA1301(1300)pCAMBIA1301-5'(ECORI)/M13RpCAMBIA1301-3'(HindIII)pCAMBIA1304pCAMBIA1301-5'(ECORI)pCAMBIA1301-3'(HindIII)pCAMBIA2300M13R(-48)M13F(-47)/M13F(-49)pCANTB5ES1S6pCAT3-enhancerRVP3此载体无反向引物pCDFDuet-1(MCSI)ACYCDuetUP1 PrimerDuetDOWN1pCDFDuet-1(MCSII
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pcambia2300载体
¥1500
