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-20℃低温保存
三年
pFGC5941
20
泽叶生物
| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pFGC5941 |
| 质粒类型: | 植物载体；RNAi载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 35S |
| 载体大小: | 11406 bp |
| 5' 测序引物及序列: | 35S promoter: CTATCCTTCGCAAGACCCTTC |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 草胺膦、草丁膦 |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 农杆菌/植物细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
LOCUS Exported 11406 bp ds-DNA circular SYN 27-JUL-2016
DEFINITION Agrobacterium binary vector for expressing dsRNA, with two multiple
cloning sites separated by an intron.
ACCESSION AY310901
VERSION .
KEYWORDS pFGC5941
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11406)
AUTHORS Kerschen A, Napoli CA, Jorgensen RA, Muller AE.
TITLE Effectiveness of RNA interference in transgenic plants.
JOURNAL FEBS Lett. 2004;566:223-8.
PUBMED 15147899
REFERENCE 2 (bases 1 to 11406)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, July 27, 2016 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT The GenBank sequence was corrected by inserting a G at position
7514.
FEATURES Location/Qualifiers
source 1..11406
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
misc_feature 1..25
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
terminator 111..363
/note="MAS terminator"
/note="mannopine synthase terminator"
CDS complement(373..924)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/note="BlpR"
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(930..1310)
/note="MAS promoter"
/note="mannopine synthase promoter (Velten et al., 1984)"
promoter 2305..2650
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_signal 2680..2734
/note="TMV Omega"
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
misc_feature 2734..2764
/note="MCS 1"
/note="multiple cloning site 1"
intron 2767..4115
/gene="chsA"
/note="chsA intron"
/note="chalcone synthase A intron from Petunia hybrida"
misc_feature 4122..4160
/note="MCS 2"
/note="multiple cloning site"
terminator 4177..4884
/note="OCS terminator"
/note="octopine synthase terminator"
misc_feature 5128..5152
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 6452..7081
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 7510..8583
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/note="pVS1 RepA"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 8649..8843
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 9187..9327
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(9513..10101)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10188..10982)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
361 aagattctgc agttagatct cggtgacggg caggaccgga cggggcggta ccggcaggct
421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
1261 taaaaactag ctcagatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
1681 cagcagtgat ccagccccaa aagagactcc tttgccccgg agattacaat ggacgatttc
1741 ctctatcttt acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc
1801 actgataatg agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
1861 agagaggcct acgcagcagg tctcatcaag acgatctacc cgagtaacaa tctccaggag
1921 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc
1981 aagaacacag agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt
2041 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
2101 cctactgaat ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact
2161 cgccgtgaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
2221 aatcttcgtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
2281 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
2341 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
2401 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
2461 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
2521 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga
2581 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
2641 ggagaggaca cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa
2701 caacaaacaa cattacaatt acatttacaa ttaccatggg gcgcgcccaa tcgatgattt
2761 aaatgtgtaa gaatttctta tgttacatta ttacattcaa cgttttatct taattggctc
2821 ttcatttgat tgaaatttga caattatttc ttgttttttt ttttgtcaca ctctttttgg
2881 gttggggtgg ccgacgaatt gtgggaaggt agaaagaggg gaggactttt gttatactcc
2941 attagtaatt actgtttccg tttcaattta tgtgacaata tttccttttt agtcggttcc
3001 aaaagaaaat gtcagcatta taaacaattt aattttgaaa ttacaatttt gccattaata
3061 aaatgattta caaccacaaa agtatctatg agcctgtttg ggtgggctta taagcagctt
3121 attttaagtg gcttataagt caaaaagtga cantttttga gaagttagaa aatcctaact
3181 tctcaaaaag tagcttttaa gccacttatg acttataagt ccaaaaattt ttaagttacc
3241 aaacatatat taatgggttt ataagcttat aagccacttt taagctcacc caaacgggtt
3301 ctatgtctca ctttagacta caaattttaa aagtcttcat ttatttctta atctccgtgg
3361 cgagtnaaac tataacacat aaagtgaaac ggagggaata agatggagtc ataaactaat
3421 ccaaatctat actctctccg ttaatttgtt ttttagtttg atttggtaca ttaataaaac
3481 agatttttcg aaggttataa acacagacag atgtttccca gcgagctagc aaaattccaa
3541 gatttctgtc gaaaattcgt gtgtttctag ctagtacttg atgttatctt taacctttta
3601 gtaatttttt gtccttttct ttctattttt catcttacaa tgaattatga gcaagttcct
3661 taagtagcat cacacgtgag atgtttttta tgatattgac taaatccaat ctttaccatt
3721 ccttaactag taaaatacaa cacatgttaa ttgatacatt gcttaacact gaggttagaa
3781 aattttagaa attagttgtc caaatgcttt gaaattagaa atctttaatc ccttattttt
3841 ttttaaaatg ttttttctca ctccaaagaa agagaaactg acatgaaagc tcaaaagatc
3901 atgaatctta ctaactttgt ggaactaaat gtacatcaga atgtttctga catgtgaaaa
3961 tgaaagctct taattttctt cttttattta ttgagggttt ttgcatgcta tgcattcaat
4021 ttgagtactt taaagcacct ataaacactt acttacactt gccttggagt ttatgtttta
4081 gtgttttctt cacatctttt ttggtcaatt tgcaggtatt tggatcctag gtgagtctag
4141 agagttaatt aagacccggg actagtccct agagtcctgc tttaatgaga tatgcgagac
4201 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc
4261 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt
4321 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt
4381 atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct
4441 atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa
4501 aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa
4561 aagtgcccca ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga
4621 agggaactcc ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc
4681 cgtccgctct accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta
4741 accgacttgc tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga
4801 agtgcaggtc aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg
4861 cgacaacatg tcgaggctca gcaggacctg caggcatgca agcttggcac tggccgtcgt
4921 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
4981 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
5041 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc
5101 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
5161 aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt
5221 ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa
5281 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
5341 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
5401 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
5461 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
5521 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
5581 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
5641 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
5701 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
5761 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
5821 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
5881 aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
5941 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
6001 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
6061 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
6121 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
6181 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
6241 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
6301 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
6361 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
6421 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
6481 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
6541 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
6601 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
6661 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
6721 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
6781 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
6841 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
6901 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
6961 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
7021 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
7081 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
7141 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
7201 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
7261 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
7321 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
7381 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
7441 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga
7501 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
7561 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca
7621 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
7681 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca
7741 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
7801 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
7861 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat
7921 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
7981 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
8041 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
8101 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
8161 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
8221 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag
8281 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
8341 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag
8401 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc
8461 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
8521 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc
8581 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt
8641 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac
8701 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat
8761 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt
8821 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa
8881 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg
8941 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa
9001 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt
9061 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
9121 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
9181 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
9241 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
9301 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
9361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
9421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
9481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
9541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
9601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
9661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
9721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
9781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
9841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
9901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
9961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
10021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
10081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
10141 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca
10201 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa
10261 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca
10321 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg
10381 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct
10441 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct
10501 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg
10561 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag
10621 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac
10681 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc
10741 cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc
10801 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat
10861 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct
10921 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc
10981 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
11041 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
11101 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
11161 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
11221 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
11281 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
11341 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
11401 ggctgg
//
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