| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pDONR-P4P1R 、pDONR P4-P1R |
| 质粒类型: | Gateway载体；DONR载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | 无 |
| 克隆方法: | Gateway；BP反应 |
| 载体大小: | 4777 bp |
| 5' 测序引物及序列: | M13/pUC fwd 5'CCCAGTCACGACGTTGTAAAACG 3' |
| 3' 测序引物及序列: | M13/pUC rev 5' AGCGGATAACAATTTCACACAGG 3' |
| 载体标签: | 无 |
| 载体抗性: | 卡那霉素，氯霉素（仅空载体） |
| 真核筛选标记: | 无 |
| 克隆菌株: | 阳性筛选用DB3.1 ，阴性筛选用OmniMAX2-T1 |
| 备注: | pDONR-P4P1R载体是MultiSite Gateway载体， 与载体一同构成三片段系统， 目标载体是pDEST-R4R3，是研究启动子的理想工具。 |
| 产品目录号: | ZY2537-023 |
| 病毒/非病毒: | 非病毒 |
- ¥2000
- ZYbscience
- 中国/美国
- ZY2537-023
- 2025年07月08日
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- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pDONR-P4P1R
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 4779 bp ds-DNA circular SYN 10-AUG-2016
DEFINITION synthetic circular DNA
KEYWORDS pDONR-P4P1R
AUTHORS Biofeng Lab.
FEATURES Location/Qualifiers
source 1..4779
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 268..295
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 537..553
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 593..824
/gene="mutant version of attP"
/bound_moiety="BP Clonase(TM)"
/note="attP4"
/note="recombination site for the Gateway(R) BP reaction"
CDS complement(1181..1486)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(1836..2489)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG"
promoter complement(2490..2592)
/note="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
protein_bind 2750..2981
/gene="mutant version of attP"
/bound_moiety="BP Clonase(TM)"
/note="attP1"
/note="recombination site for the Gateway(R) BP reaction
(pDONR(TM)201 version)"
promoter complement(3021..3039)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3044..3060)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 3173..3982
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 4129..4717
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
661 ataatgccaa ctttgtatag aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat
721 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatg
781 cagtcactat gaatcaacta cttagatggt attagtgacc tgtagaattc gagctcgacc
841 gacagccttc caaatgttct tctcaaacgg aatcgtcgta tccagcctac tcgctattgt
901 cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa gaggtgcgag cctctttttt
961 gtgtgacaaa ataaaaacat ctacctattc atatacgcta gtgtcatagt cctgaaaatc
1021 atctgcatca agaacaattt cacaactctt atacttttct cttacaagtc gttcggcttc
1081 atctggattt tcagcctcta tacttactaa acgtgataaa gtttctgtaa tttctactgt
1141 atcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
1201 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
1261 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
1321 tatgcaccac cgggtaaagt tcacgggaga ctttatccga cagcagacgt gcactggcca
1381 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
1441 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg
1501 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc cgtcccttcc
1561 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
1621 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
1681 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata
1741 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct
1801 ggcttttagt aagccggatc cacgcggcgt ttacgccccc cctgccactc atcgcagtac
1861 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
1921 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
1981 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
2041 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
2101 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
2161 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
2221 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
2281 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
2341 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
2401 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
2461 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
2521 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
2581 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
2641 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
2701 caaagtgcgt cgggtgatgc tgccaactta gtcgacgctc tagagctcca aataatgatt
2761 ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata
2821 atgccaactt tgtacaaaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata
2881 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatgcag
2941 tcactatgaa ccaactactt agatggtatt agtgacctgt agaattcgag ctctagagct
3001 gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
3061 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
3121 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
3181 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
3241 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
3301 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
3361 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
3421 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
3481 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
3541 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
3601 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
3661 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
3721 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
3781 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
3841 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
3901 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
3961 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
4021 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
4081 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
4141 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
4201 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
4261 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
4321 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
4381 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
4441 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
4501 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
4561 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
4621 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
4681 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
4741 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
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pDONR-P4P1R载体
¥2000
