pCL-Eco载体

pCL-Eco载体

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  • ZYbscience
  • 中国/美国
  • ZY12371
  • 2025年07月16日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      pCL-Eco

    • 库存：

      20

    • 供应商：

      泽叶生物

    载体基本信息

    出品公司: ZYbscience
    试剂名称: pCL-Eco
    质粒类型: 哺乳动物载体；逆病毒包装；单质粒包装系统
    高拷贝/低拷贝: 高拷贝
    克隆方法: 限制性内切酶，多克隆位点
    启动子: CMV
    载体大小: 12341 bp
    5' 测序引物及序列: CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
    3' 测序引物及序列: --
    载体标签: 无
    载体抗性: Ampicillin
    筛选标记: 无
    克隆菌株: DH5α等
    宿主细胞（系）: 包装细胞系如293T
    备注: 逆病毒包装载体pCL-Eco是单质粒包装系统，快速产生Helper-free、毒力高的逆病毒颗粒。
    产品目录号: ZY12371
    稳定性: 瞬表达
    组成型/诱导型: 组成型
    病毒/非病毒: 非病毒

    载体质粒图谱和多克隆位点信息

    pCL-Eco载体图谱


    载体简介

    逆病毒包装载体pCL-Eco的使用方法

293T Growth Medium:

   450 ml DMEM (JRH Biosciences)
   50 ml FBS (JRH Sciences)
   5 ml Glutamine (200 mM in saline, JRH Sciences)
   2.5 ml Gentamycin (10 mg/ml in water, Sigma)

HC11 Growth Medium:

  5 ml L-Glutamine (200 mM in saline, JRH Sciences)
  2.5 ml Bovine Insulin (1 mg/ml in 0.01 N HCL, Sigma)
  2.5 ml Gentamycin (10 mg/ml in water, Sigma)
  0.5 ml EGF (10 μg/ml in water, Gibco)
  50 ml FBS (JRH Sciences)
  440 ml RPMI (JRH Sciences)


Day 0: Split 293T cells for transfection:

  Wash cells off plate with 10 ml Hanks and put into 15 ml Falcon tube
  Add 1 ml 10X trypsin and mix by inversion for about 1-2 minutes
  Spin at 1000 rpm in clinical centrifuge for 1-2 minutes
  Resuspend cells in 10 ml growth medium
  Count cells
  Plate cells at 1X106 cells per 100 mm dish


Day 2: Transfect 293T cells:

  Plan transfection using 2 μg total DNA and 12 μl FuGene in 200 μl total 
  volume per 100 mm dish
  Dilute Fugene: 12 μl per reaction in serum free DMEM for total volume of
  200 μl

  Mix DNA plasmids in polypropelene tubes (Falcon #2063):
     1 μg DNA of interest
     1 μg pCL-Eco
     Add 200 μl diluted FuGene dropwise to each DNA tube

  Incubate RT 15 min
  Feed cells with 5 ml fresh growth media and add FuGene/DNA mixture to media
 

Day 3: Change medium:

  • Remove viral medium and CAREFULLY feed with 6 ml growth medium
  This step may reduce a cytostatic factor (produced after transfections) that
  can inhibit growth and infection of your target cells.

  Split HC11 cells for infection
  Seed HC11 cells at 300,000 cells per 100 mm dish
  If using coverslips, incubate with FBS for at least 1 hour prior to plating


Day 4: Infect target (HC11) cells:

  • Collect virus-containing media off 293T cells
  • Syringe filter media through .45 μm filter onto target cells
   (Evaporation O/N and loss in filter leaves about 5 ml per plate)
  • Add 10 μl 5mg/ml polybrene (final conc = 10 μg polybrene/ml media)
   (Store polybrene in aliquots to decrease freeze/thaw cycles)
  • Wrap plates in parafilm
  • Spin cells in clinical centrifuge 10 min at 1800 rpm
  • Rotate plate 1/3. Spin 10 min at 1800 rpm
  • Rotate plate 1/3. Spin 10 min at 1800 rpm
  • Remove viral media and replace with fresh growth media


Day 6+: Harvest HC11 cells:

  • Wash cells with HBSS
  • Scrape remaining cells, pellet and flash freeze for protein/RNA analysis

  Stain cells for beta-gal expression:

  Fixing Solution :
    1.35 ml 37% Formaldehyde
    0.2 ml Gluteraldehyde
    1X PBS to 25 mls

  Staining Solution:
    625μl 40 mg/ml X-gal in DMF
    50 μl 1M MgCL2
    750 μl 100 mM Potassium Ferricyanide
    750 μl 100 mM Potassium Ferrocyanide
    1X PBS to 25 mls
    [100 mM Potassium Ferrocyanide = 2.11 g/50ml PBS]
    [100 mM Potassium Ferricyanide = 1.64 g/50 ml PBS]

  Store at room temperature, protect from light.
    1. Make -gal solution and warm in 37°C water bath to prevent crystallization
    2. Aspirate off media
    3. Wash cells 1X PBS
    4. Fix cells 5 min at RT in fixing solution
    5. Wash 3X PBS
    6 Optional: Add PBS to cells and warm plate at 37°C 5 min (reduces
      crystallization)
    7. Stain cells with staining solution at 37°C for 1-24 hrs
    8. Store stained cells in 70% EtOH at 4°C
      (EMBO J. 5: pg 3133, 1986)


Note:

   HC11 and MEC primary culture cells are very adherent and can withstand
   the force of spinning the plates at 1800 rpm. We found that HC11 cells died at
   2200 rpm. However, this is cell type-dependent, and spinning speed should be
   optimized accordingly.
   Expect to see -gal staining at the periphery of the plate. During the spin
   infection the media is pushed to the outside. The cells in the center of the plate
   will not be exposed to virus and will not be efficiently infected with retrovirus.

    载体序列

    LOCUS       pCL-Eco Sequencing Result	12341 bp 	DNA	SYN
DEFINITION  pCL-Eco Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..12341
                     /organism="pCL-Eco Sequencing Result"
                     /mol_type="other DNA"
     rep_origin      267..886
                     /label="pBR322_origin"
     misc_feature    complement(1160..1179)
                     /label="SV40pro_F_primer"
     rep_origin      complement(1164..1241)
                     /label="SV40_origin"
     terminator      complement(1709..1828)
                     /label="SV40_PA_terminator"
     misc_feature    complement(1723..1742)
                     /label="EBV_rev_primer"
     misc_feature    complement(2253..2300)
                     /label="SV40_3_splice"
     misc_feature    complement(2306..2321)
                     /label="SV40_int"
     misc_feature    2540..2562
                     /label="pGEX_3_primer"
     CDS             complement(3033..5030)
                     /label="ORF frame 1"
                     /translation="MARSTLSKPLKNKVNPRGPLIPLILLMLRGVSTASPGSSPHQVY
                     NITWEVTNGDRETVWATSGNHPLWTWWPDLTPDLCMLAHHGPSYWGLEYQSPFSSPPG
                     PPCCSGGSSPGCSRDCEEPLTSLTPRCNTAWNRLKLDQTTHKSNEGFYVCPGPHRPRE
                     SKSCGGPDSFYCAYWGCETTGRAYWKPSSSWDFITVNNNLTSDQAVQVCKDNKWCNPL
                     VIRFTDAGRRVTSWTTGHYWGLRLYVSGQDPGLTFGIRLRYQNLGPRVPIGPNPVLAD
                     QQPLSKPKPVKSPSVTKPPSGTPLSPTQLPPAGTENRLLNLVDGAYQALNLTSPDKTQ
                     ECWLCLVAGPPYYEGVAVLGTYSNHTSAPANCSVASQHKLTLSEVTGQGLCIGAVPKT
                     HQALCNTTQTSSRGSYYLVAPTGTMWACSTGLTPCISTTILNLTTDYCVLVELWPRVT
                     YHSPSYVYGLFERSNRHKREPVSLTLALLLGGLTMGGIAAGIGTGTTALMATQQFQQL
                     QAAVQDDLREVEKSISNLEKSLTSLSEVVLQNRRGLDLLFLKEGGLCAALKEECCFYA
                     DHTGLVRDSMAKLRERLNQRQKLFESTQGWFEGLFNRSPWFTTLISTIMGPLIVLLMI
                     LLFGPCILNRLVQFVKDRISVVQALVLTQQYHQLKPIEYEP*"
     CDS             complement(4970..8242)
                     /label="ORF frame 2"
                     /translation="MGPMGQPLQVLTLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAW
                     AETGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQ
                     SPWNTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVL
                     DLKDAFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDL
                     ADFRIQHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQ
                     VKYLGYLLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAP
                     LYPLTKTGTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVL
                     TQKLGPWRRPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHA
                     VEALVKQPPDRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLD
                     ILAEAHGTRPDLTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAG
                     TSAQRAELIALTQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGLLTSEGKEIKN
                     KDEILALLKALFLPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLL
                     IENSSPYTSEHFHYTVTDIKDLTKLGAIYDKTKKYWVYQGKPVMPDQFTFELLDFLHQ
                     LTHLSFSKMKALLERSHSPYYMLNRDRTLKNITETCKACAQVNASKSAVKQGTRVRGH
                     RPGTHWEIDFTEIKPGLYGYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPR
                     FGMPQVLGTDNGPAFVSKVSQTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTK
                     LTLATGSRDWVLLLPLALYRARNTPGPHGLTPYEILYGAPPPLVNFPDPDMTRVTNSP
                     SLQAHLQALYLVQHEVWRPLAAAYQEQLDRPVVPHPYRVGDTVWVRRHQTKNLEPRWK
                     GPYTVLLTTPTALKVDGIAAWIHAAHVKAADPGGGPSSRLTWRVQRSQNPLKIRLTRE
                     AP*"
     misc_feature    complement(5079..5095)
                     /label="pBMN_5_primer"
                     /translation="MGPMGQPLQVLTLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAW
                     AETGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQ
                     SPWNTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVL
                     DLKDAFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDL
                     ADFRIQHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQ
                     VKYLGYLLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAP
                     LYPLTKTGTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVL
                     TQKLGPWRRPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHA
                     VEALVKQPPDRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLD
                     ILAEAHGTRPDLTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAG
                     TSAQRAELIALTQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGLLTSEGKEIKN
                     KDEILALLKALFLPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLL
                     IENSSPYTSEHFHYTVTDIKDLTKLGAIYDKTKKYWVYQGKPVMPDQFTFELLDFLHQ
                     LTHLSFSKMKALLERSHSPYYMLNRDRTLKNITETCKACAQVNASKSAVKQGTRVRGH
                     RPGTHWEIDFTEIKPGLYGYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPR
                     FGMPQVLGTDNGPAFVSKVSQTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTK
                     LTLATGSRDWVLLLPLALYRARNTPGPHGLTPYEILYGAPPPLVNFPDPDMTRVTNSP
                     SLQAHLQALYLVQHEVWRPLAAAYQEQLDRPVVPHPYRVGDTVWVRRHQTKNLEPRWK
                     GPYTVLLTTPTALKVDGIAAWIHAAHVKAADPGGGPSSRLTWRVQRSQNPLKIRLTRE
                     AP*"
     CDS             complement(8570..10186)
                     /label="ORF frame 2"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWP
                     TFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTE
                     DPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLR
                     AGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGT
                     LLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYR
                     QLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETN
                     VSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEE
                     KEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCK
                     EKGHWAKDCPKKPRGPRGPRPQTSLLTLDD*"
     misc_feature    complement(9770..10177)
                     /label="gag"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWP
                     TFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTE
                     DPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLR
                     AGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGT
                     LLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYR
                     QLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETN
                     VSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEE
                     KEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCK
                     EKGHWAKDCPKKPRGPRGPRPQTSLLTLDD*"
     misc_feature    complement(9783..9799)
                     /label="pBABE_5_primer"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWP
                     TFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTE
                     DPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLR
                     AGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGT
                     LLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYR
                     QLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETN
                     VSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEE
                     KEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCK
                     EKGHWAKDCPKKPRGPRGPRPQTSLLTLDD*"
     misc_feature    complement(9815..9837)
                     /label="MSCV_primer"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWP
                     TFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTE
                     DPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLR
                     AGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGT
                     LLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYR
                     QLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETN
                     VSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEE
                     KEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCK
                     EKGHWAKDCPKKPRGPRGPRPQTSLLTLDD*"
     misc_feature    complement(9815..9837)
                     /label="pLXSN_5_primer"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWP
                     TFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTE
                     DPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLR
                     AGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGT
                     LLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYR
                     QLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETN
                     VSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEE
                     KEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCK
                     EKGHWAKDCPKKPRGPRGPRPQTSLLTLDD*"
     misc_feature    complement(10319..11018)
                     /label="5_LTR"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWP
                     TFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTE
                     DPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLR
                     AGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGT
                     LLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYR
                     QLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETN
                     VSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEE
                     KEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCK
                     EKGHWAKDCPKKPRGPRGPRPQTSLLTLDD*"
     promoter        complement(10497..11077)
                     /label="CMV_immearly_promoter"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWP
                     TFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTE
                     DPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLR
                     AGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGT
                     LLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYR
                     QLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETN
                     VSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEE
                     KEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCK
                     EKGHWAKDCPKKPRGPRGPRPQTSLLTLDD*"
     misc_feature    complement(10524..10544)
                     /label="CMV_fwd_primer"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWP
                     TFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTE
                     DPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLR
                     AGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGT
                     LLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYR
                     QLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETN
                     VSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEE
                     KEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCK
                     EKGHWAKDCPKKPRGPRGPRPQTSLLTLDD*"
     misc_feature    complement(10710..10997)
                     /label="CAG_enhancer"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWP
                     TFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTE
                     DPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLR
                     AGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGT
                     LLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYR
                     QLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETN
                     VSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEE
                     KEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCK
                     EKGHWAKDCPKKPRGPRGPRPQTSLLTLDD*"
     misc_feature    11342..11364
                     /label="pGEX_3_primer"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWP
                     TFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTE
                     DPPPYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLR
                     AGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGT
                     LLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYR
                     QLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETN
                     VSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEE
                     KEERRRTEDEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCK
                     EKGHWAKDCPKKPRGPRGPRPQTSLLTLDD*"
     promoter        11523..11551
                     /label="AmpR_promoter"

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    公司名称
    报价日期
    载体pCL-Eco
    ¥800
    上海沪震实业有限公司
    2025年07月14日询价
    pCL-Eco载体
    ¥1000
    上海再康生物科技有限公司
    2025年11月21日询价
    pCL-Eco载体
    ¥1000
    上海信裕生物科技有限公司
    2025年07月13日询价
    pCL-Ampho载体
    ¥1250
    上海泽叶生物科技有限公司
    2025年07月14日询价
    pCL-Ampho载体
    ￥1200
    上海盖宁生物科技有限公司
    2025年11月18日询价
    pCL-Eco载体
    ¥1250