| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pLP1 |
| 质粒类型: | 哺乳动物细胞慢病毒包装载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8889 bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 备注: | 载体使用Stbl3感受态细胞扩增质粒，可以减少载体基因重组几率。pLP/VSVG, pLP1, pLP2载体是Invitrogen公司出品的配套使用的慢病毒包装载体。 第三代慢病毒包装系统组成成份有pLP1、pLP2和pLP/VSVG（或者pCMV-VSVG）。在使用的时候，按照pLP1(3):pLP2(2):pLP/VSVG(3)的比例配成1 ug/ul的第三代慢病毒包装系统package Mix。转染时，对于60mm的细胞培养皿来说，按照慢病毒基因质粒（3ug）和package mix（6 ug，也就是6 ul）的比例进行慢病毒质粒包装实验。 |
| 产品目录号: | K4970-00 |
| 稳定性: | / |
| 组成型: | -- |
| 病毒/非病毒: | 慢病毒 |
- ¥1500
- ZYbscience
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- ZY
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pLP1
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLP1 8889 bp DNA circular SYN
DEFINITION pLP1
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..8889
/organism="pLP1"
/mol_type="other DNA"
promoter 80..656
/label="CMV_immearly_promoter"
misc_feature 159..446
/label="CAG_enhancer"
misc_feature 613..633
/label="CMV_fwd_primer"
promoter 614..683
/label="CMV_promoter"
promoter 626..745
/label="CMV2_promoter"
misc_feature 657..676
/label="pCEP_fwd_primer"
misc_feature 659..683
/label="LNCX_primer"
misc_feature 933..1316
/label="bGlob_int"
gene 1355..2857
/label="Gag_HIV (variant)"
/gene="Gag_HIV (variant)"
CDS 1355..2857
/label="ORF frame 2"
CDS 2923..5661
/label="ORF frame 1"
misc_feature 5350..5365
/label="cPPT"
CDS 5606..6124
/label="ORF frame 2"
misc_feature 5686..5919
/label="RRE"
gene complement(7813..8673)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(7813..8673)
/label="ORF frame 1"
promoter complement(8715..8743)
/label="AmpR_promoter"
misc_feature complement(8847..8866)
/label="SV40pro_F_primer"
ORIGIN
1 TTGGCCCATT GCATACGTTG TATCCATATC ATAATATGTA CATTTATATT GGCTCATGTC
61 CAACATTACC GCCATGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG
121 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
181 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
241 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
301 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
361 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
421 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA
481 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG
541 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT
601 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG
661 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA
721 GAAGACACCG GGACCGATCC AGCCTCCCCT CGAAGCTTAC ATGTGGTACC GAGCTCGGAT
781 CCTGAGAACT TCAGGGTGAG TCTATGGGAC CCTTGATGTT TTCTTTCCCC TTCTTTTCTA
841 TGGTTAAGTT CATGTCATAG GAAGGGGAGA AGTAACAGGG TACACATATT GACCAAATCA
901 GGGTAATTTT GCATTTGTAA TTTTAAAAAA TGCTTTCTTC TTTTAATATA CTTTTTTGTT
961 TATCTTATTT CTAATACTTT CCCTAATCTC TTTCTTTCAG GGCAATAATG ATACAATGTA
1021 TCATGCCTCT TTGCACCATT CTAAAGAATA ACAGTGATAA TTTCTGGGTT AAGGCAATAG
1081 CAATATTTCT GCATATAAAT ATTTCTGCAT ATAAATTGTA ACTGATGTAA GAGGTTTCAT
1141 ATTGCTAATA GCAGCTACAA TCCAGCTACC ATTCTGCTTT TATTTTATGG TTGGGATAAG
1201 GCTGGATTAT TCTGAGTCCA AGCTAGGCCC TTTTGCTAAT CATGTTCATA CCTCTTATCT
1261 TCCTCCCACA GCTCCTGGGC AACGTGCTGG TCTGTGTGCT GGCCCATCAC TTTGGCAAAG
1321 CACGTGAGAT CTGAATTCGA GATCTGCCGC CGCCATGGGT GCGAGAGCGT CAGTATTAAG
1381 CGGGGGAGAA TTAGATCGAT GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA
1441 TAAATTAAAA CATATAGTAT GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG
1501 CCTGTTAGAA ACATCAGAAG GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA
1561 GACAGGATCA GAAGAACTTA GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA
1621 TCAAAGGATA GAGATAAAAG ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA
1681 CAAAAGTAAG AAAAAAGCAC AGCAAGCAGC AGCTGACACA GGACACAGCA ATCAGGTCAG
1741 CCAAAATTAC CCTATAGTGC AGAACATCCA GGGGCAAATG GTACATCAGG CCATATCACC
1801 TAGAACTTTA AATGCATGGG TAAAAGTAGT AGAAGAGAAG GCTTTCAGCC CAGAAGTGAT
1861 ACCCATGTTT TCAGCATTAT CAGAAGGAGC CACCCCACAA GATTTAAACA CCATGCTAAA
1921 CACAGTGGGG GGACATCAAG CAGCCATGCA AATGTTAAAA GAGACCATCA ATGAGGAAGC
1981 TGCAGAATGG GATAGAGTGC ATCCAGTGCA TGCAGGGCCT ATTGCACCAG GCCAGATGAG
2041 AGAACCAAGG GGAAGTGACA TAGCAGGAAC TACTAGTACC CTTCAGGAAC AAATAGGATG
2101 GATGACACAT AATCCACCTA TCCCAGTAGG AGAAATCTAT AAAAGATGGA TAATCCTGGG
2161 ATTAAATAAA ATAGTAAGAA TGTATAGCCC TACCAGCATT CTGGACATAA GACAAGGACC
2221 AAAGGAACCC TTTAGAGACT ATGTAGACCG ATTCTATAAA ACTCTAAGAG CCGAGCAAGC
2281 TTCACAAGAG GTAAAAAATT GGATGACAGA AACCTTGTTG GTCCAAAATG CGAACCCAGA
2341 TTGTAAGACT ATTTTAAAAG CATTGGGACC AGGAGCGACA CTAGAAGAAA TGATGACAGC
2401 ATGTCAGGGA GTGGGGGGAC CCGGCCATAA AGCAAGAGTT TTGGCTGAAG CAATGAGCCA
2461 AGTAACAAAT CCAGCTACCA TAATGATACA GAAAGGCAAT TTTAGGAACC AAAGAAAGAC
2521 TGTTAAGTGT TTCAATTGTG GCAAAGAAGG GCACATAGCC AAAAATTGCA GGGCCCCTAG
2581 GAAAAAGGGC TGTTGGAAAT GTGGAAAGGA AGGACACCAA ATGAAAGATT GTACTGAGAG
2641 ACAGGCTAAT TTTTTAGGGA AGATCTGGCC TTCCCACAAG GGAAGGCCAG GGAATTTTCT
2701 TCAGAGCAGA CCAGAGCCAA CAGCCCCACC AGAAGAGAGC TTCAGGTTTG GGGAAGAGAC
2761 AACAACTCCC TCTCAGAAGC AGGAGCCGAT AGACAAGGAA CTGTATCCTT TAGCTTCCCT
2821 CAGATCACTC TTTGGCAGCG ACCCCTCGTC ACAATAAAGA TAGGGGGGCA ATTAAAGGAA
2881 GCTCTATTAG ATACAGGAGC AGATGATACA GTATTAGAAG AAATGAATTT GCCAGGAAGA
2941 TGGAAACCAA AAATGATAGG GGGAATTGGA GGTTTTATCA AAGTAAGACA GTATGATCAG
3001 ATACTCATAG AAATCTGCGG ACATAAAGCT ATAGGTACAG TATTAGTAGG ACCTACACCT
3061 GTCAACATAA TTGGAAGAAA TCTGTTGACT CAGATTGGCT GCACTTTAAA TTTTCCCATT
3121 AGTCCTATTG AGACTGTACC AGTAAAATTA AAGCCAGGAA TGGATGGCCC AAAAGTTAAA
3181 CAATGGCCAT TGACAGAAGA AAAAATAAAA GCATTAGTAG AAATTTGTAC AGAAATGGAA
3241 AAGGAAGGAA AAATTTCAAA AATTGGGCCT GAAAATCCAT ACAATACTCC AGTATTTGCC
3301 ATAAAGAAAA AAGACAGTAC TAAATGGAGA AAATTAGTAG ATTTCAGAGA ACTTAATAAG
3361 AGAACTCAAG ATTTCTGGGA AGTTCAATTA GGAATACCAC ATCCTGCAGG GTTAAAACAG
3421 AAAAAATCAG TAACAGTACT GGATGTGGGC GATGCATATT TTTCAGTTCC CTTAGATAAA
3481 GACTTCAGGA AGTATACTGC ATTTACCATA CCTAGTATAA ACAATGAGAC ACCAGGGATT
3541 AGATATCAGT ACAATGTGCT TCCACAGGGA TGGAAAGGAT CACCAGCAAT ATTCCAGTGT
3601 AGCATGACAA AAATCTTAGA GCCTTTTAGA AAACAAAATC CAGACATAGT CATCTATCAA
3661 TACATGGATG ATTTGTATGT AGGATCTGAC TTAGAAATAG GGCAGCATAG AACAAAAATA
3721 GAGGAACTGA GACAACATCT GTTGAGGTGG GGATTTACCA CACCAGACAA AAAACATCAG
3781 AAAGAACCTC CATTCCTTTG GATGGGTTAT GAACTCCATC CTGATAAATG GACAGTACAG
3841 CCTATAGTGC TGCCAGAAAA GGACAGCTGG ACTGTCAATG ACATACAGAA ATTAGTGGGA
3901 AAATTGAATT GGGCAAGTCA GATTTATGCA GGGATTAAAG TAAGGCAATT ATGTAAACTT
3961 CTTAGGGGAA CCAAAGCACT AACAGAAGTA GTACCACTAA CAGAAGAAGC AGAGCTAGAA
4021 CTGGCAGAAA ACAGGGAGAT TCTAAAAGAA CCGGTACATG GAGTGTATTA TGACCCATCA
4081 AAAGACTTAA TAGCAGAAAT ACAGAAGCAG GGGCAAGGCC AATGGACATA TCAAATTTAT
4141 CAAGAGCCAT TTAAAAATCT GAAAACAGGA AAGTATGCAA GAATGAAGGG TGCCCACACT
4201 AATGATGTGA AACAATTAAC AGAGGCAGTA CAAAAAATAG CCACAGAAAG CATAGTAATA
4261 TGGGGAAAGA CTCCTAAATT TAAATTACCC ATACAAAAGG AAACATGGGA AGCATGGTGG
4321 ACAGAGTATT GGCAAGCCAC CTGGATTCCT GAGTGGGAGT TTGTCAATAC CCCTCCCTTA
4381 GTGAAGTTAT GGTACCAGTT AGAGAAAGAA CCCATAATAG GAGCAGAAAC TTTCTATGTA
4441 GATGGGGCAG CCAATAGGGA AACTAAATTA GGAAAAGCAG GATATGTAAC TGACAGAGGA
4501 AGACAAAAAG TTGTCCCCCT AACGGACACA ACAAATCAGA AGACTGAGTT ACAAGCAATT
4561 CATCTAGCTT TGCAGGATTC GGGATTAGAA GTAAACATAG TGACAGACTC ACAATATGCA
4621 TTGGGAATCA TTCAAGCACA ACCAGATAAG AGTGAATCAG AGTTAGTCAG TCAAATAATA
4681 GAGCAGTTAA TAAAAAAGGA AAAAGTCTAC CTGGCATGGG TACCAGCACA CAAAGGAATT
4741 GGAGGAAATG AACAAGTAGA TAAATTGGTC AGTGCTGGAA TCAGGAAAGT ACTATTTTTA
4801 GATGGAATAG ATAAGGCCCA AGAAGAACAT GAGAAATATC ACAGTAATTG GAGAGCAATG
4861 GCTAGTGATT TTAACCTACC ACCTGTAGTA GCAAAAGAAA TAGTAGCCAG CTGTGATAAA
4921 TGTCAGCTAA AAGGGGAAGC CATGCATGGA CAAGTAGACT GTAGCCCAGG AATATGGCAG
4981 CTAGATTGTA CACATTTAGA AGGAAAAGTT ATCTTGGTAG CAGTTCATGT AGCCAGTGGA
5041 TATATAGAAG CAGAAGTAAT TCCAGCAGAG ACAGGGCAAG AAACAGCATA CTTCCTCTTA
5101 AAATTAGCAG GAAGATGGCC AGTAAAAACA GTACATACAG ACAATGGCAG CAATTTCACC
5161 AGTACTACAG TTAAGGCCGC CTGTTGGTGG GCGGGGATCA AGCAGGAATT TGGCATTCCC
5221 TACAATCCCC AAAGTCAAGG AGTAATAGAA TCTATGAATA AAGAATTAAA GAAAATTATA
5281 GGACAGGTAA GAGATCAGGC TGAACATCTT AAGACAGCAG TACAAATGGC AGTATTCATC
5341 CACAATTTTA AAAGAAAAGG GGGGATTGGG GGGTACAGTG CAGGGGAAAG AATAGTAGAC
5401 ATAATAGCAA CAGACATACA AACTAAAGAA TTACAAAAAC AAATTACAAA AATTCAAAAT
5461 TTTCGGGTTT ATTACAGGGA CAGCAGAGAT CCAGTTTGGA AAGGACCAGC AAAGCTCCTC
5521 TGGAAAGGTG AAGGGGCAGT AGTAATACAA GATAATAGTG ACATAAAAGT AGTGCCAAGA
5581 AGAAAAGCAA AGATCATCAG GGATTATGGA AAACAGATGG CAGGTGATGA TTGTGTGGCA
5641 AGTAGACAGG ATGAGGATTA ACACATGGAA TTCCGGAGCG GCCGCAGGAG CTTTGTTCCT
5701 TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA
5761 GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA
5821 GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT
5881 CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG
5941 AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA
6001 ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG
6061 CTTCCGCGGA ATTCACCCCA CCAGTGCAGG CTGCCTATCA GAAAGTGGTG GCTGGTGTGG
6121 CTAATGCCCT GGCCCACAAG TATCACTAAG CTCGCTTTCT TGCTGTCCAA TTTCTATTAA
6181 AGGTTCCTTT GTTCCCTAAG TCCAACTACT AAACTGGGGG ATATTATGAA GGGCCTTGAG
6241 CATCTGGATT CTGCCTAATA AAAAACATTT ATTTTCATTG CAATGATGTA TTTAAATTAT
6301 TTCTGAATAT TTTACTAAAA AGGGAATGTG GGAGGTCAGT GCATTTAAAA CATAAAGAAA
6361 TGAAGAGCTA GTTCAAACCT TGGGAAAATA CACTATATCT TAAACTCCAT GAAAGAAGGT
6421 GAGGCTGCAA ACAGCTAATG CACATTGGCA ACAGCCCCTG ATGCCTATGC CTTATTCATC
6481 CCTCAGAAAA GGATTCAAGT AGAGGCTTGA TTTGGAGGTT AAAGTTTTGC TATGCTGTAT
6541 TTTACATTAC TTATTGTTTT AGCTGTCCTC ATGAATGTCT TTTCACTACC CATTTGCTTA
6601 TCCTGCATCT CTCAGCCTTG ACTCCACTCA GTTCTCTTGC TTAGAGATAC CACCTTTCCC
6661 CTGAAGTGTT CCTTCCATGT TTTACGGCGA GATGGTTTCT CCTCGCCTGG CCACTCAGCC
6721 TTAGTTGTCT CTGTTGTCTT ATAGAGGTCT ACTTGAAGAA GGAAAAACAG GGGGCATGGT
6781 TTGACTGTCC TGTGAGCCCT TCTTCCCTGC CTCCCCCACT CACAGTGACC CGGAATCCCT
6841 CGACATGGCA GTCTAGCACT AGTGCGGCCG CAGATCTGCT TCCTCGCTCA CTGACTCGCT
6901 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
6961 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
7021 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
7081 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
7141 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
7201 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
7261 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
7321 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
7381 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
7441 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
7501 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
7561 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
7621 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
7681 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
7741 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
7801 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
7861 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
7921 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
7981 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
8041 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
8101 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
8161 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
8221 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
8281 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
8341 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
8401 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
8461 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
8521 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
8581 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
8641 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
8701 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
8761 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGGGA TCCCCTGAGG
8821 GGGCCCCCAT GGGCTAGAGG ATCCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA
8881 GCCATGAGC
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文献和实验相关实验
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zhangyuxianggx 各位大侠好！现有pcDNA3-mCherry和pGEM-hgene两个东西在滤纸上，老师就说让构建载体，具体是什么意思啊？我理解的是一个真核表达载体和构建好的pGEM质粒，但是不知下一步该怎么做？恳请各位好心的大侠指点一二。 shylook 直接插入cDNA吧 zhangyuxianggx 那个pGEM就是已经插入了DNA的载体。请问这两个东西溶下来以后
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pLP1载体
¥1500
