LOCUS pAAV-minCMV-mCherry 4887 bp DNA SYNDEFINITION pAAV-minCMV-mCherryCOMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT ORIGDB|GenBankCOMMENT VNTAUTHORNAME|biofeng.com|COMMENT VNTNAME|pAAV-minCMV-mCherry|FEATURES Location/Qualifiers source 1..4887 /organism="pAAV-minCMV-mCherry" /mol_type="other DNA" promoter 163..181 /label="T7_promoter" misc_feature 233..252 /label="pCEP_fwd_primer" misc_feature 235..259 /label="LNCX_primer" CDS 279..989 /label="ORF frame 3" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ YERAEGRHSTGGMDELYK*" gene 282..986 /label="mCherry" /gene="mCherry" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ YERAEGRHSTGGMDELYK*" misc_feature 459..512 /label="Q66M (mCherry)" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ YERAEGRHSTGGMDELYK*" misc_feature 1016..1603 /label="WPRE" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ YERAEGRHSTGGMDELYK*" CDS 1104..1715 /label="ORF frame 3" /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK SSSFPWLLAYVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS LWAASPHRYRALLERSTGGIPVTPPQCLSWPWKLPLQCPPALS*" CDS 1117..1650 /label="ORF frame 1" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" terminator 1632..2110 /label="hGH_PA_terminator" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" rep_origin 2382..2688 /label="f1_origin" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" misc_feature 2956..2978 /label="pGEX_3_primer" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" promoter 3137..3165 /label="AmpR_promoter" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" gene 3207..4067 /label="Ampicillin" /gene="Ampicillin" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" CDS 3207..4067 /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 4222..4841 /label="pBR322_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtATCTA GATAATACGA CTCACTATAG 181 GGGGATCCAC GTATGTCGAG GTAGGCGTGT ACGGTGGGAG GCCTATATAA GCAGAGCTCG 241 TTTAGTGAAC CGTCAGATCG CCTGGAGGTA CCGCCACCAT GGTGAGCAAG GGCGAGGAGG 301 ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA 361 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA 421 CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC 481 CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT 541 TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG 601 GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA 661 AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT 721 GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC 781 AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG 841 CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT 901 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA 961 CCGGCGGCAT GGACGAGCTG TACAAGTAAG AATTCgatat caagcttatc gataatcaac 1021 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 1081 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 1141 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 1201 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 1261 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 1321 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 1381 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg 1441 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 1501 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 1561 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc 1621 tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga 1681 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc 1741 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca 1801 agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt 1861 ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca 1921 gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt 1981 ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt 2041 gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt 2101 ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta 2161 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 2221 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc 2281 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 2341 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg 2401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 2461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 2521 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 2581 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 2641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 2701 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 2761 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt 2821 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 2881 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 2941 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 3001 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 3061 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 3121 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 3181 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 3241 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 3301 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 3361 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 3421 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 3481 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 3541 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 3601 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 3661 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 3721 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 3781 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 3841 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 3901 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 3961 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 4021 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 4081 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 4141 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 4201 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 4261 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 4321 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 4381 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 4441 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 4501 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 4561 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 4621 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 4681 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 4741 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 4801 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 4861 ccttttgctg gccttttgct cacatgt