| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pIEXBac1, pIEXBac-1, pIEX/Bac1, pIEX/Bac-1 |
| 质粒类型: | 昆虫细胞表达载体；蛋白纯化 |
| 启动子: | Polyhedrin |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6796 bp |
| 5' 测序引物: | -- |
| 3' 测序引物: | -- |
| 载体标签: | C-His, N-EK, N-Strep |
| 载体抗性: | Ampicillin 氨苄 |
| 备注: |
拥有hr5增强子和IE1启动子；双标签系统可以采用两种纯化方案。
|
| 产品目录号: | ZY71724-3 |
| 稳定性: | 瞬转 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 杆状病毒 |
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- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pIEX/Bac-1
- 库存：
20
- 供应商：
泽叶生物
pIEXBac1载体基本信息
pIEXBac1载体质粒图谱和多克隆位点信息
pIEXBac1载体简介
The dual-purpose pIEx/Bac™ vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants. Transient transfection and early baculovirus expression is driven by a promoter/enhancer combination that recruits endogenous insect cell transcription machinery, the AcNPV derived hr5 enhancer and ie1 promoter. Late/very late expression in the baculovirus mode is driven by the strong p10 promoter. The pIEx/Bac-1 vector carries an N-terminal Strep•Tag II coding sequence (1) followed by a recognition site for enterokinase.
The multiple cloning region is followed by an optional C-terminal His•Tag® coding sequence. The presence of two “gentle elution" tags at both the N- and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map.
pIEXBac1载体序列
LOCUS pIEx/Bac-1 6796 bp DNA circular 17-OCT-2006
DEFINITION pIEx/BAC hr5/ie1/p10 Strep-Tag II/EkLIC/10xHis.
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
misc_recomb complement(2916..4334)
/dnas_title="1629"
/vntifkey=86
/label=1629
rep_origin 6271..6726
/dnas_title="f1 origin"
/vntifkey=33
/label=f1 origin
CDS complement(5282..6139)
/dnas_title="Ap"
/vntifkey=4
/label=Ap
rep_origin complement(4521..4521)
/dnas_title="origin"
/vntifkey=33
/label=origin
misc_recomb 4..453
/dnas_title="lef-2"
/vntifkey=86
/label=lef-2
misc_recomb complement(491..1096)
/dnas_title="ORF 603"
/vntifkey=86
/label=ORF 603
rep_origin 4354..5113
/dnas_title="pUC Ori"
/vntifkey=33
/label=pUC Ori
enhancer 1196..1677
/dnas_title="hr5"
/vntifkey=9
/label=hr5
polyA_signal 2742..2747
/dnas_title="PolyA signal"
/vntifkey=25
/label=PolyA signal
5'UTR 2229..2278
/dnas_title="ie1 5'-UTR"
/vntifkey=52
/label=ie1 5'-UTR
promoter 2279..2391
/dnas_title="p10 promoter + 5'UTR"
/vntifkey=29
/label=p10 promoter + 5'UTR
promoter 1682..2228
/dnas_title="ie1 promoter"
/vntifkey=29
/label=ie1 promoter
terminator 2565..2871
/dnas_title="ie1 Terminator"
/vntifkey=43
/label=ie1 Terminator
protein_bind 2433..2447
/dnas_title="Ek site"
/vntifkey=31
/label=Ek site
protein_bind 2403..2426
/dnas_title="Strep-Tag II"
/vntifkey=31
/label=Strep-Tag II
misc_binding 2517..2546
/dnas_title="10x His•Tag"
/vntifkey=20
/label=10x His•Tag
BASE COUNT 1824 a 1462 c 1506 g 2004 t
ORIGIN
1 GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG
61 AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC
121 AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT
181 GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG
241 GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC
301 CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT
361 AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC
421 TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT
481 TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA
541 TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC
601 AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT
661 CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT
721 ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT
781 TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT
841 TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT
901 TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA
961 TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT
1021 TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT
1081 TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA
1141 TCAATATATA GTTGCTGATC AGATCTGATC ATGGAGATAA TTAAAATGAT AAGGGCGCGT
1201 AAAACACAAT CAAGTATGAG TCATAAGCTG ATGTCATGTT TTGCACACGG CTCATAACCG
1261 AACTGGCTTT ACGAGTAGAA TTCTACTTGT AACGCACGAT CAGTGGATGA TGTCATTTGT
1321 TTTTCAAATC GAGATGATGT CATGTTTTGC ACACGGCTCA TAAACTCGCT TTACGAGTAG
1381 AATTCTACGT GTAACGCACG ATCGATTGAT GAGTCATTTG TTTTGCAATA TGATATCATA
1441 CAATATGACT CATTTGTTTT TCAAAACCGA ACTTGATTTA CGGGTAGAAT TCTACTTGTA
1501 AAGCACAATC AAAAAGATGA TGTCATTTGT TTTTCAAAAC TGAACTCGCT TTACGAGTAG
1561 AATTCTACGT GTAAAACACA ATCAAGAAAT GATGTCATTT GTTATAAAAA TAAAAGCTGA
1621 TGTCATGTTT TGCACATGGC TCATAACTAA ACTCGCTTTA CGGGTAGAAT TCTACGCGCG
1681 TCGATGTCTT TGTGATGCGC GCGACATTTT TGTAGGTTAT TGATAAAATG AACGGATACG
1741 TTGCCCGACA TTATCATTAA ATCCTTGGCG TAGAATTTGT CGGGTCCATT GTCCGTGTGC
1801 GCTAGCATGC CCGTAACGGA CCTCGTACTT TTGGCTTCAA AGGTTTTGCG CACAGACAAA
1861 ATGTGCCACA CTTGCAGCTC TGCATGTGTG CGCGTTACCA CAAATCCCAA CGGCGCAGTG
1921 TACTTGTTGT ATGCAAATAA ATCTCGATAA AGGCGCGGCG CGCGAATGCA GCTGATCACG
1981 TACGTCCCTC GTGTTCCGTT CAAGGACGGT GTTATCGACC TCAGATTAAT GTTTATCGGC
2041 CGACTGTTTT CGTATCCGCT CACCAAACGC GTTTTTGCAT TAACATTGTA TGTCGGCGGA
2101 TGTTCTATAT CTAATTTGAA TAAATAAACG ATAACCGCGT TGGTTTTAGA GGGCATAATA
2161 AAAGAAATAT TGTTATCGTG TTCGCCATTA GGGCAGTATA AATTGACGTT CATGTTGGAT
2221 ATTGTTTCAG TTGCAAGTTG ACACTGGCGG CGACAAGATC GTGAACAACC AAGTGACTCC
2281 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC
2341 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCCATGGCAA
2401 GCTGGAGCCA CCCGCAGTTC GAAAAGGGTG CAGATGACGA CGACAAGGTA CCGGATCCGA
2461 CGTCGGTCGA CAAGCTTGCG GCCGCAGGAG CTCCGGGCTT CTCCTCAATT TCCGCTCATC
2521 ACCACCATCA TCACCATCAC CACCACTAAG TGATTAACCT CAGGTTATAC ATATATTTTG
2581 AATTTAATTA ATTATACATA TATTTTATAT TATTTTTGTC TTTTATTATC GAGGGGCCGT
2641 TGTTGGTGTG GGGTTTTGCA TAGAAATAAC AATGGGAGTT GGCGACGTTG CTGCGCCAAC
2701 ACCACCTCCC TTCCCTCCTT TCATCATGTA TCTGTAGATA AAATAAAATA TTAAACCTAA
2761 AAACAAGACC GCGCCTATCA ACAAAATGAT AGGCATTAAC TTGCCGCTGA CGCTGTCACT
2821 AACGTTGGAC GATTTGCCGA CTAAACCTTC ATCGCCCAGT AACCAATCTA GGTAGCTGAG
2881 CGCATGCAAG CTGATCCGGG TTATTAGTAC ATTTATTAAG CGCTAGATTC TGTGCGTTGT
2941 TGATTTACAG ACAATTGTTG TACGTATTTT AATAATTCAT TAAATTTATA ATCTTTAGGG
3001 TGGTATGTTA GAGCGAAAAT CAAATGATTT TCAGCGTCTT TATATCTGAA TTTAAATATT
3061 AAATCCTCAA TAGATTTGTA AAATAGGTTT CGATTAGTTT CAAACAAGGG TTGTTTTTCC
3121 GAACCGATGG CTGGACTATC TAATGGATTT TCGCTCAACG CCACAAAACT TGCCAAATCT
3181 TGTAGCAGCA ATCTAGCTTT GTCGATATTC GTTTGTGTTT TGTTTTGTAA TAAAGGTTCG
3241 ACGTCGTTCA AAATATTATG CGCTTTTGTA TTTCTTTCAT CACTGTCGTT AGTGTACAAT
3301 TGACTCGACG TAAACACGTT AAATAGAGCT TGGACATATT TAACATCGGG CGTGTTAGCT
3361 TTATTAGGCC GATTATCGTC GTCGTCCCAA CCCTCGTCGT TAGAAGTTGC TTCCGAAGAC
3421 GATTTTGCCA TAGCCACACG ACGCCTATTA ATTGTGTCGG CTAACACGTC CGCGATCAAA
3481 TTTGTAGTTG AGCTTTTTGG AATTATTTCT GATTGCGGGC GTTTTTGGGC GGGTTTCAAT
3541 CTAACTGTGC CCGATTTTAA TTCAGACAAC ACGTTAGAAA GCGATGGTGC AGGCGGTGGT
3601 AACATTTCAG ACGGCAAATC TACTAATGGC GGCGGTGGTG GAGCTGATGA TAAATCTACC
3661 ATCGGTGGAG GCGCAGGCGG GGCTGGCGGC GGAGGCGGAG GCGGAGGTGG TGGCGGTGAT
3721 GCAGACGGCG GTTTAGGCTC AAATGTCTCT TTAGGCAACA CAGTCGGCAC CTCAACTATT
3781 GTACTGGTTT CGGGCGCCGT TTTTGGTTTG ACCGGTCTGA GACGAGTGCG ATTTTTTTCG
3841 TTTCTAATAG CTTCCAACAA TTGTTGTCTG TCGTCTAAAG GTGCAGCGGG TTGAGGTTCC
3901 GTCGGCATTG GTGGAGCGGG CGGCAATTCA GACATCGATG GTGGTGGTGG TGGTGGAGGC
3961 GCTGGAATGT TAGGCACGGG AGAAGGTGGT GGCGGCGGTG CCGCCGGTAT AATTTGTTCT
4021 GGTTTAGTTT GTTCGCGCAC GATTGTGGGC ACCGGCGCAG GCGCCGCTGG CTGCACAACG
4081 GAAGGTCGTC TGCTTCGAGG CAGCGCTTGG GGTGGTGGCA ATTCAATATT ATAATTGGAA
4141 TACAAATCGT AAAAATCTGC TATAAGCATT GTAATTTCGC TATCGTTTAC CGTGCCGATA
4201 TTTAACAACC GCTCAATGTA AGCAATTGTA TTGTAAAGAG ATTGTCTCAA GCTCGGATCG
4261 ATCCCGCACG CCGATAACAA GCCTTTTCAT TTTTACTACA GCATTGTAGT GGCGAGACAC
4321 TTCGCTGTCG TCGAGGTTTA AACGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
4381 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
4441 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
4501 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
4561 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
4621 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
4681 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
4741 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
4801 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
4861 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
4921 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
4981 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
5041 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
5101 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
5161 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
5221 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
5281 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
5341 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
5401 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
5461 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
5521 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
5581 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
5641 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
5701 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
5761 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
5821 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
5881 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
5941 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
6001 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
6061 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
6121 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
6181 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
6241 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG
6301 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC
6361 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA
6421 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC
6481 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT
6541 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA
6601 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT
6661 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA
6721 CAATTTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
6781 CTTCGCTATT ACGCCA
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文献和实验相关实验
齐全的辅助产品，包括Strep•Tag II融合标签单抗、HRP 耦联单抗、携带 Strep•Tag II 标签的蛋白Marker 各类携带Strep•Tag II融合标签的载体特性 pET-51b(+) pET-52b(+) pIEx-8 pIEx-9 pIEx-10 pTriEx-5 pTriEx-6 pTriEx-7 宿主系统及启动子E.coliT7lac启动子 √ √ √ √ √ 昆虫 细胞e1/hr5√ √ √ 昆虫细 胞10启动子√ √ √ 哺乳动物CMVie启动子/ 增强子
在目的基因起始密码子ATG前加上这一个序列 pVL1393， 昆虫表达载体 pIEX/Bac-1 昆虫表达载体 pTET on pTeT off pTER-tight pBAD HisA amp pBAD myc HisA amp pACYC184 amp pBluescript II SK (+) amp pIRESneo3 pPICZ α B pVL1393， pIEX/Bac-1 pcmv-myc P2
包含足够多的克隆，来保证文库的代表性。一般使用如下的经验公式来确定： N = ln (1-P ) / ln (1-f ) P是希望得到的覆盖率，f是插入片段大小与基因组DNA大小的比值，N是所需的克隆数。 举例来说，用BAC载体构建人类基因组文库，人类基因组大小为3 x 109 bp, 插入片段大小为100kb，希望覆盖率99%，那么所需要的BAC克隆数为 N = ln (1 - 0.99) / ln (1 - [106bp / 3 x 109 bp
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pIEX/Bac-1载体
¥1350
