| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | psiCHECK-2 |
| 质粒类型: | 哺乳动物细胞表达系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6273bp |
| 5' 测序引物及序列: | n/a |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | ZY8021 |
| 稳定性: | 瞬表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
psiCHECK 2
- 库存：
20
- 供应商：
泽叶生物
psiCHECK2载体基本信息
psiCHECK2载体质粒图谱和多克隆位点信息
psiCHECK2载体简介
psiCHECK2载体序列
LOCUS psiCHECK-2 6273 bp DNA SYN
DEFINITION psiCHECK-2
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6273
/organism="psiCHECK-2"
/mol_type="other DNA"
misc_feature complement(85..105)
/label="pBABE_3_primer"
misc_feature complement(91..306)
/label="SV40_enhancer"
promoter 103..371
/label="SV40_promoter"
rep_origin 270..347
/label="SV40_origin"
misc_feature 332..351
/label="SV40pro_F_primer"
misc_feature 489..621
/label="chim_int"
promoter 666..684
/label="T7_promoter"
CDS 694..1629
/label="ORF frame 1"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*"
terminator 1688..1736
/label="synth_PA_terminator"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*"
CDS 2069..2524
/label="ORF frame 2"
/translation="MGTVYVDRLWSIRTTAVILPETFCGTSRVTRLTRSVRWATNTRT
GHRYTILSPGGARDCRSFREWRSCFIPVARCSRLLAVSPEEIYLHVFSSMMTQTPPSV
LSLANSNTQMQSGRRGPRSTSHIKVTRVASNTERPCSDPLKSLAFRYCW*"
promoter complement(2269..2417)
/label="TATA_promoter"
/translation="MGTVYVDRLWSIRTTAVILPETFCGTSRVTRLTRSVRWATNTRT
GHRYTILSPGGARDCRSFREWRSCFIPVARCSRLLAVSPEEIYLHVFSSMMTQTPPSV
LSLANSNTQMQSGRRGPRSTSHIKVTRVASNTERPCSDPLKSLAFRYCW*"
promoter 2280..2495
/label="TK_promoter"
/translation="MGTVYVDRLWSIRTTAVILPETFCGTSRVTRLTRSVRWATNTRT
GHRYTILSPGGARDCRSFREWRSCFIPVARCSRLLAVSPEEIYLHVFSSMMTQTPPSV
LSLANSNTQMQSGRRGPRSTSHIKVTRVASNTERPCSDPLKSLAFRYCW*"
CDS complement(2493..3527)
/label="ORF frame 2"
/translation="MKALGHGFAHLLRQWGAARGNLVQVRQVVLVDQGALSEEAEQGW
HQQGRLNFVVLQAAQEQLLLEAVHQHHPKAANQVAQGGEHAEAVVEWHHAQNSGVGDD
LVAENRVAGVREAHTGGAMRQGHALRQAGRPRAVHDQGNGFVPVERLWHEVVLVEARR
QMRCHECVHALEALVVGLRVHDDDLLDDRQLLLHVQDLLQALLRHEHHGRLRNAHAVQ
QLALVVNVVSRGHGHSDEQGAQHWHEELQRVLRAHHDAVVGVQAVPLHGLGQAHRHLE
VLGIGDVHLNVGIGEGNGARHQGIPLHGLVQLLASGAILQRVEGSRALLNVLSIGHGG
FTNSTGMPSF*"
CDS 2532..4184
/label="ORF frame 3"
/translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
terminator complement(4225..4344)
/label="SV40_PA_terminator"
/translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
misc_feature complement(4239..4258)
/label="EBV_rev_primer"
/translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
promoter 4517..4545
/label="AmpR_promoter"
/translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
gene 4587..5447
/label="Ampicillin"
/gene="Ampicillin"
/translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
CDS 4587..5447
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 5602..6221
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT
61 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG
121 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG
181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG
241 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC
301 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG
361 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
421 AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA
481 CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT
541 TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC
601 ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA
661 GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG
721 CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG
781 CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT
841 CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC
901 GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT
961 GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC
1021 CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC
1081 TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG
1141 ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA
1201 GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG
1261 ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG
1321 GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC
1381 AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT
1441 CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA
1501 GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG
1561 GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC
1621 GAGCAGTAAT TCTAGGCGAT CGCTCGAGCC CGGGAATTCG TTTAAACCTA GAGCGGCCGC
1681 TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAGGA
1741 TCTAAATGAG TCTTCGGACC TCGCGGGGGC CGCTTAAGCG GTGGTTAGGG TTTGTCTGAC
1801 GCGGGGGGAG GGGGAAGGAA CGAAACACTC TCATTCGGAG GCGGCTCGGG GTTTGGTCTT
1861 GGTGGCCACG GGCACGCAGA AGAGCGCCGC GATCCTCTTA AGCACCCCCC CGCCCTCCGT
1921 GGAGGCGGGG GTTTGGTCGG CGGGTGGTAA CTGGCGGGCC GCTGACTCGG GCGGGTCGCG
1981 CGCCCCAGAG TGTGACCTTT TCGGTCTGCT CGCAGACCCC CGGGCGGCGC CGCCGCGGCG
2041 GCGACGGGCT CGCTGGGTCC TAGGCTCCAT GGGGACCGTA TACGTGGACA GGCTCTGGAG
2101 CATCCGCACG ACTGCGGTGA TATTACCGGA GACCTTCTGC GGGACGAGCC GGGTCACGCG
2161 GCTGACGCGG AGCGTCCGTT GGGCGACAAA CACCAGGACG GGGCACAGGT ACACTATCTT
2221 GTCACCCGGA GGCGCGAGGG ACTGCAGGAG CTTCAGGGAG TGGCGCAGCT GCTTCATCCC
2281 CGTGGCCCGT TGCTCGCGTT TGCTGGCGGT GTCCCCGGAA GAAATATATT TGCATGTCTT
2341 TAGTTCTATG ATGACACAAA CCCCGCCCAG CGTCTTGTCA TTGGCGAATT CGAACACGCA
2401 GATGCAGTCG GGGCGGCGCG GTCCCAGGTC CACTTCGCAT ATTAAGGTGA CGCGTGTGGC
2461 CTCGAACACC GAGCGACCCT GCAGCGACCC GCTTAAAAGC TTGGCATTCC GGTACTGTTG
2521 GTAAAGCCAC CATGGCCGAT GCTAAGAACA TTAAGAAGGG CCCTGCTCCC TTCTACCCTC
2581 TGGAGGATGG CACCGCTGGC GAGCAGCTGC ACAAGGCCAT GAAGAGGTAT GCCCTGGTGC
2641 CTGGCACCAT TGCCTTCACC GATGCCCACA TTGAGGTGGA CATCACCTAT GCCGAGTACT
2701 TCGAGATGTC TGTGCGCCTG GCCGAGGCCA TGAAGAGGTA CGGCCTGAAC ACCAACCACC
2761 GCATCGTGGT GTGCTCTGAG AACTCTCTGC AGTTCTTCAT GCCAGTGCTG GGCGCCCTGT
2821 TCATCGGAGT GGCCGTGGCC CCTGCTAACG ACATTTACAA CGAGCGCGAG CTGCTGAACA
2881 GCATGGGCAT TTCTCAGCCT ACCGTGGTGT TCGTGTCTAA GAAGGGCCTG CAGAAGATCC
2941 TGAACGTGCA GAAGAAGCTG CCTATCATCC AGAAGATCAT CATCATGGAC TCTAAGACCG
3001 ACTACCAGGG CTTCCAGAGC ATGTACACAT TCGTGACATC TCATCTGCCT CCTGGCTTCA
3061 ACGAGTACGA CTTCGTGCCA GAGTCTTTCG ACAGGGACAA AACCATTGCC CTGATCATGA
3121 ACAGCTCTGG GTCTACCGGC CTGCCTAAGG GCGTGGCCCT GCCTCATCGC ACCGCCTGTG
3181 TGCGCTTCTC TCACGCCCGC GACCCTATTT TCGGCAACCA GATCATCCCC GACACCGCTA
3241 TTCTGAGCGT GGTGCCATTC CACCACGGCT TCGGCATGTT CACCACCCTG GGCTACCTGA
3301 TTTGCGGCTT TCGGGTGGTG CTGATGTACC GCTTCGAGGA GGAGCTGTTC CTGCGCAGCC
3361 TGCAAGACTA CAAAATTCAG TCTGCCCTGC TGGTGCCAAC CCTGTTCAGC TTCTTCGCTA
3421 AGAGCACCCT GATCGACAAG TACGACCTGT CTAACCTGCA CGAGATTGCC TCTGGCGGCG
3481 CCCCACTGTC TAAGGAGGTG GGCGAAGCCG TGGCCAAGCG CTTTCATCTG CCAGGCATCC
3541 GCCAGGGCTA CGGCCTGACC GAGACAACCA GCGCCATTCT GATTACCCCA GAGGGCGACG
3601 ACAAGCCTGG CGCCGTGGGC AAGGTGGTGC CATTCTTCGA GGCCAAGGTG GTGGACCTGG
3661 ACACCGGCAA GACCCTGGGA GTGAACCAGC GCGGCGAGCT GTGTGTGCGC GGCCCTATGA
3721 TTATGTCCGG CTACGTGAAT AACCCTGAGG CCACAAACGC CCTGATCGAC AAGGACGGCT
3781 GGCTGCACTC TGGCGACATT GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC
3841 GCCTGAAGTC TCTGATCAAG TACAAGGGCT ACCAGGTGGC CCCAGCCGAG CTGGAGTCTA
3901 TCCTGCTGCA GCACCCTAAC ATTTTCGACG CCGGAGTGGC CGGCCTGCCC GACGACGATG
3961 CCGGCGAGCT GCCTGCCGCC GTCGTCGTGC TGGAACACGG CAAGACCATG ACCGAGAAGG
4021 AGATCGTGGA CTATGTGGCC AGCCAGGTGA CAACCGCCAA GAAGCTGCGC GGCGGAGTGG
4081 TGTTCGTGGA CGAGGTGCCC AAGGGCCTGA CCGGCAAGCT GGACGCCCGC AAGATCCGCG
4141 AGATCCTGAT CAAGGCTAAG AAAGGCGGCA AGATCGCCGT GTAATAATTC TAGAGTCGGG
4201 GCGGCCGGCC GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA
4261 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG
4321 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC
4381 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA
4441 AAATCGATAA GGATCCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT
4501 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC
4561 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC
4621 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA
4681 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG
4741 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG
4801 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC
4861 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA
4921 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG
4981 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA
5041 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC
5101 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT
5161 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG
5221 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA
5281 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA
5341 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA
5401 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG
5461 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG
5521 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT
5581 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG
5641 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC
5701 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA
5761 CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA
5821 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC
5881 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG
5941 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC
6001 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG
6061 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT
6121 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT
6181 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
6241 CCTTTTGCTG GCCTTTTGCT CACATGGCTC GAC
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文献和实验相关实验
1.常用载体分类：2,常用载体元件介绍：(1) 启动子：启动子是位于结构基因5'端上游的DNA序列，能活化RNA聚合酶，使之与模板DNA准确的结合并具有转录起始的特异性。启动子主要分为广谱启动子和特异性启动子。广谱启动子为在各种组织、细胞上广泛表达的启动子；特异性启动子为在特异性组织或者细胞类型上表达的启动子。常用广谱启动子有CAG，CMV，EF1a，PGK等，还包含启动RNA的III型启动子U6和H1启动子；常用特异性启动子如成熟神经元特异性启动子hSyn，投射神经元特异性启动
恰当的选择固相载体和连接分子是固相反应的重要组成部分. 固相载体和连接分子都应该在多种遗传反应过程期间是保持稳定, 而且产品的切除应该是在温和的条件下实现. 本章将介绍各种不同的载体和连接分子. 如果反应后可以用过滤的方法去除, 从理论上讲, 任何材料都可用作固相载体. 聚乙烯, 聚苯乙烯-外涂聚乙烯薄层, 平滑的纸或棉花等都可以. 最常用的载体是: 聚苯乙烯(PS), 聚乙二醇(PEG), Tentagel (TG), 和PEGA (聚乙二醇-聚丙
zhangyuxianggx 各位大侠好！现有pcDNA3-mCherry和pGEM-hgene两个东西在滤纸上，老师就说让构建载体，具体是什么意思啊？我理解的是一个真核表达载体和构建好的pGEM质粒，但是不知下一步该怎么做？恳请各位好心的大侠指点一二。 shylook 直接插入cDNA吧 zhangyuxianggx 那个pGEM就是已经插入了DNA的载体。请问这两个东西溶下来以后
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psiCHECK 2载体
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