双向启动子载体pBI-CMV2表达绿色荧光蛋白AcGFP1 The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest). Expression is driven by constitutively active bidirectional human cytomegalovirus promoters (PminCMV). The gene(s) of interest must contain an initiation codon and a stop codon. eatures Constitutively express a protein of interest and a reporter protein (or two proteins of interest) pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.
LOCUS pBI-CMV2 3822 bp DNA SYNDEFINITION pBI-CMV2ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..3822 /organism="pBI-CMV2" /mol_type="other DNA" promoter 4..568 /label="CMV_immearly_promoter" misc_feature 71..358 /label="CAG_enhancer" misc_feature 525..545 /label="CMV_fwd_primer" promoter 526..595 /label="CMV_promoter" gene complement(1799..2659) /label="Ampicillin" /gene="Ampicillin" CDS complement(1799..2659) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(2701..2729) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" CDS 2969..3520 /label="ORF frame 2" /translation="MADYDPLDCSLRRSGPSLVQLIHAVGDGGGGHEAEVDHVIALLV GVLGQGALGGQVVVIGQQHRAIADGGILLVVVGQLHAAILDVVADLEVHLDAILGLVG HDVHIVGVVVVLHLIAQDVAILLEIGAGQLDAIHQGIALELHLGARLVVAVILEEDGA LLDVALRHGALEEVVLLHVIGVA*" CDS complement(3017..3736) /label="ORF frame 3" /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MIYFGFVTAAAITHGMDELYK*" gene complement(3020..3733) /label="AcGFP1" /gene="AcGFP1" /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MIYFGFVTAAAITHGMDELYK*" misc_feature complement(3756..3780) /label="LNCX_primer" /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MIYFGFVTAAAITHGMDELYK*" misc_feature complement(3763..3782) /label="pCEP_fwd_primer" /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MIYFGFVTAAAITHGMDELYK*"ORIGIN 1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat 661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc 901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca 2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg 2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa 2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta 2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct 3001 caggagatct gggccctcac ttgtacagct catccatgcc gtgggtgatg gcggcggcgg 3061 tcacgaagcc gaagtagatc atgtgatcgc gcttctcgtt ggggtccttg gacagggcgc 3121 tctgggtgga caggtagtgg ttatcgggca gcagcacagg gccatcgccg atgggggtat 3181 tctgctggta gtggtcggcc agctgcacgc tgccatcctc gatgttgtgg cggatcttga 3241 agttcacctt gatgccattc ttggccttgt cggtcatgat gtacacattg tgggcgttgt 3301 agttgtactc catcttattg cccaggatgt tgccatcctc cttgaaatcg gtgccggtca 3361 gctcgatgcg attcaccagg gtatcgccct cgaacttcac ctcggcgcgc gacttgtagt 3421 tgccgtcatc ctcgaagaag atggtgcgct cctggatgta gccctcaggc atggcgctct 3481 tgaagaagtc gtgctgcttc atgtgatcgg ggtagcgtga gaagcactgc acgccgtagc 3541 tcagggtggt caccagggtg ggccagggca caggcagctt gccggtggtg cagatgaact 3601 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccgctcacg ctgaacttgt 3661 ggccattcac atcgccattc agctcgatca ggatgggcac gatgccggtg aacagctcgg 3721 cgcccttgct caccatggtg gcgaattctc caggcgatct gacggttcac taaacgagct 3781 ctgcttatat aggcctccca ccgtacacgc cacctcgaca ta//