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pEBVHisC载体

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  • ZYbscience
  • 中国/美国
  • ZY
  • 2025年06月26日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      pEBVHisC

    • 库存：

      20

    • 供应商：

      泽叶生物

    载体基本信息

    出品公司: ZYbscience
    载体名称: pEBVHisC
    质粒类型: 哺乳动物表达载体；附加体载体
    高拷贝/低拷贝: 高拷贝
    克隆方法: 限制性内切酶，多克隆位点
    启动子: RSV
    载体大小: 10029 bp
    5' 测序引物及序列: pREP Forward 5’ GCTCGATACAATAAACGCC 3’
    3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
    载体标签: His tag (N-term)
    载体抗性: 氨苄青霉素
    筛选标记: Hygromycin
    克隆菌株: DH5α 等
    宿主细胞（系）: 人类细胞；灵长类细胞；犬类细胞
    备注: pEBVHis-lacZ
    产品目录号: --
    稳定性: 稳表达
    组成型/诱导型: 组成型
    病毒/非病毒: 非病毒

    载体质粒图谱和多克隆位点信息

    pEBVHisC载体图谱



    pEBVHisC多克隆位点

    pEBVHis载体特征

    载体简介

    载体序列

    LOCUS       pEBVHisC	10315 bp 	DNA	SYN
DEFINITION  pEBVHisC
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="pEBVHisC"
                     /mol_type="other DNA"
     misc_feature    112..134
                     /label="pGEX_3_primer"
     terminator      279..410
                     /label="SV40_PA_terminator"
     misc_feature    367..386
                     /label="EBV_rev_primer"
     misc_feature    complement(506..529)
                     /label="Xpress_EK"
     misc_feature    complement(533..565)
                     /label="T7_leader"
     misc_feature    complement(548..566)
                     /label="Xpress_fwd_primer"
     misc_feature    complement(632..650)
                     /label="pREP_fwd_primer"
     CDS             1211..1948
                     /label="ORF frame 2"
                     /translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
                     NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
                     HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
                     RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
                     VPAPDRTIASHRPCAQAASSKLPSTKL*"
     terminator      complement(1469..1739)
                     /label="TK_PA_terminator"
                     /translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
                     NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
                     HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
                     RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
                     VPAPDRTIASHRPCAQAASSKLPSTKL*"
     CDS             complement(1747..2772)
                     /label="ORF frame 2"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            complement(1750..2757)
                     /label="hygroB"
                     /gene="hygroB"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     CDS             2014..3384
                     /label="ORF frame 1"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     promoter        2891..3039
                     /label="TATA_promoter"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     gene            3046..3384
                     /label="tet (852 - 1190)"
                     /gene="tet (852 - 1190)"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
                     FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
                     QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
                     QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
                     SRATST*"
     CDS             3288..3899
                     /label="ORF frame 3"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
                     TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
                     LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
                     IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
     gene            complement(4333..5193)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
                     TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
                     LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
                     IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
     CDS             complement(4333..5193)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5235..5263)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             complement(5819..7033)
                     /label="ORF frame 1"
                     /translation="MAFYLEGACAVETRMMMTDYWDSWASFLHVHDLSPWLFHDFPPW
                     LFHVLYPGGLHYLLDPGLHYLLDPGLHCLLDPGLHLLLLPLLLLPLLLLLPLLPLLLL
                     PLLPLLLLPLLPLLLLPLLPLLLLLPLLPLLLLLPLLPLLLLPLLPLLLLPLLPLLLL
                     PLLLLPLLLLPLLLLPLLPLLPLLLLLPLLLLPLLPLLPLLLLPLLLLLPLLPLLPLL
                     LLLPLLPLLLLLPLLLLLPLLPLLPLLLLLPLLPLLLLLPLLLLLPLLPLLPLLLLLP
                     LLLLLPLLPLLPLLPLLLLLPLLLLLPLLLLPLPLLLLLLFHRGSLCSQCNLDVFGVS
                     GHHLYVLALILSRPGLLVFRLLVLVLFPVLVHGYHPLFFEVHCRRSLLVQMCLPSLLG
                     HFQVLYLAPRQT*"
     CDS             5821..7746
                     /label="ORF frame 1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRG
                     RGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGG
                     AGAGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAG
                     GGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGA
                     GAGGGAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAG
                     GAGAGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGS
                     GGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSG
                     SPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGP
                     RGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVF
                     VYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFL
                     QTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDG
                     DDGDEGGDGDEGEEGQE*"
     CDS             5936..7048
                     /label="ORF frame 2"
                     /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
                     KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
                     GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
                     EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
                     GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
                     EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
                     VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
     rep_origin      8050..10220
                     /label="EBV_origin"
                     /translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
                     KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
                     GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
                     EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
                     GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
                     EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
                     VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
ORIGIN
    1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
   61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
  121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
  181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
  241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
  301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
  361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
  421 tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
  481 gatctcgagg tcggatccat cgatccttat cgtcatcgtc gtacagatcc cgacccattt
  541 gctgtccacc agtcatgcta gccataccat gatgatgatg atgatgagaa ccccccatgg
  601 aggtgcacac caatgtggtg aatggtcaaa tggcgtttat tgtatcgagc taggcactta
  661 aatacaatta tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacctgtc
  721 tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg
  781 tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac
  841 aagtgtattg cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc
  901 tgaggggact cctaaccgcg tacaaccgaa gccccgctgg gcgcctaaac acaccctagt
  961 cccctcagat acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
 1021 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
 1081 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
 1141 cggcagcaga gcagattgta ctgagagtgc accatacaag ctcaggatct gcgatgataa
 1201 gctgtcaaac atgagaattg gtcgaccact gggcgccaga aatccgcgcg gtggtttttg
 1261 ggggtcgggg gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg
 1321 gtccatgccc aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggaccag
 1381 accccacgca acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga
 1441 ccccgtccct aacccacggg gccagtggct atggcagggc ctgccgcccc gacgttggct
 1501 gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg
 1561 gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga
 1621 accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc
 1681 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca
 1741 tctcccctat tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
 1801 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
 1861 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
 1921 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
 1981 cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
 2041 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
 2101 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
 2161 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
 2221 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
 2281 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
 2341 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
 2401 cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc
 2461 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
 2521 gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
 2581 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
 2641 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
 2701 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
 2761 aggctttttc atatctcatt gcccgggatc tgcggcacgc tgttgacgct gttaagcggg
 2821 tcgctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg cgaagtggac
 2881 ctgggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga caagacgctg
 2941 ggcggggttt gtgtcatcat agaactaaag acatgcaaat atatttcttc cggggacacc
 3001 gccagcaaac gcgagcaacg ggccacgggg atgaagcagg gcatggcggc cgacgcgctg
 3061 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
 3121 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
 3181 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
 3241 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
 3301 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
 3361 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
 3421 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
 3481 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc agcaaaaggc
 3541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
 3601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata

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    产品名称
    产品价格
    公司名称
    报价日期
    pLVX-Puro载体
    ￥750
    上海盖宁生物科技有限公司
    2026年03月19日询价
    HEK-293T-ACE2过表达细胞株(HEK-293T- ACE2 Cell Line)
    ￥18500
    翌圣生物科技（上海）股份有限公司
    2026年01月23日询价
    lentiCRISPR v2载体
    ¥1000
    上海泽叶生物科技有限公司
    2025年07月16日询价
    载体pTRV2
    ¥800
    上海沪震实业有限公司
    2025年07月16日询价
    pGADT7载体
    ¥1000
    上海信裕生物科技有限公司
    2025年07月16日询价
    pEBVHisC载体
    ¥3000