| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCMV-SPORT2 |
| 质粒类型: | 哺乳动物表达载体；cDNA克隆载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 4774 bp |
| 5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
| 3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: | 用于cDNA文库的构建和表达 |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 2025年07月09日
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- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pCMV-SPORT2
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-Sport2 4474 bp DNA SYN
DEFINITION pCMV-Sport2
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4474
/organism="pCMV-Sport2"
/mol_type="other DNA"
terminator complement(135..254)
/label="SV40_PA_terminator"
misc_feature complement(149..168)
/label="EBV_rev_primer"
misc_feature complement(542..557)
/label="SV40_int"
misc_feature 649..671
/label="M13_pUC_fwd_primer"
promoter 664..680
/label="M13_forward20_primer"
promoter 691..709
/label="T7_promoter"
misc_feature complement(833..850)
/label="Sp6_primer"
misc_feature complement(886..908)
/label="M13_pUC_rev_primer"
promoter complement(932..1051)
/label="CMV2_promoter"
misc_feature complement(994..1018)
/label="LNCX_primer"
promoter complement(994..1065)
/label="CMV_promoter"
misc_feature complement(1001..1020)
/label="pCEP_fwd_primer"
promoter complement(1017..1521)
/label="CMV_immearly_promoter"
misc_feature complement(1044..1064)
/label="CMV_fwd_primer"
misc_feature complement(1230..1517)
/label="CAG_enhancer"
misc_feature complement(1541..1563)
/label="M13_pUC_rev_primer"
promoter 1754..1782
/label="AmpR_promoter"
rep_origin complement(2054..2673)
/label="pBR322_origin"
misc_feature 2735..2768
/label="loxP"
gene complement(2870..3730)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2870..3730)
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin complement(4150..4456)
/label="f1_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagatctcc cagtcacgac
661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg agagagctat
721 gacgtcgcat gcacgcgtaa gcttgggccc ctcgagggat cctctagagc ggccgccgac
781 tagtgagctc gtcgacccgg gaattccgga ccggtacctg caggcgtacc ttctatagtg
841 tcacctaaat agctttttgc aaaagcctag tggtcatagc tgtttcctgt gtgaaattgt
901 tatccgctcc gcggcctagg ctagagtccg gaggctggat cggtcccggt gtcttctatg
961 gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct
1021 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
1081 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
1141 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
1201 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
1261 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
1321 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
1381 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
1441 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
1501 atttaccgta agttatgtaa cgacctgcac gatgctgttt cctgtgtgaa attgttatcc
1561 gctcacaatt ccacacatta tacgagccgg aagctataaa gtgtaaagcc tggggtgcct
1621 aatgagtgaa agggcctcgt atacgcctat ttttataggt taatgtcatg ataataatgg
1681 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
1741 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
1801 aataatattg aaaaacgcgc gaattgcaag ctctgcatta atgaatcggc caacgcgcgg
1861 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
1921 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
1981 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
2041 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
2101 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
2161 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
2221 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
2281 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
2341 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
2401 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
2461 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
2521 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
2581 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
2641 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
2701 acgaaaactc acgttaaggg attttggtca tgccataact tcgtatagca tacattatac
2761 gaagttatgg catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
2821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
2881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
2941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
3001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
3061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
3121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
3181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
3241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
3301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
3361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
3421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
3481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
3541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
3601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
3661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
3721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
3781 tgccaggggt gggcacacat atttgatacc agcgatccct acacagcaca taattcaatg
3841 cgacttccct ctatcgcaca tcttagacct ttattctccc tccagcacac atcgaagctg
3901 ccgagcaagc cgttctcacc agtccaagac ctggcatgag cggatacata tttgaatgta
3961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
4021 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
4081 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
4141 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
4201 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
4261 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
4321 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
4381 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
4441 ccgccgcgct taatgcgccg ctacagggcg cgtc
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pCMV-SPORT2载体
¥2000
