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pET-24b(+)载体

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  • ZYbscience
  • 中国/美国
  • ZY69750-3
  • 2026年01月08日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      -20℃低温保存

    • 保质期

      三年

    • 英文名

      pET-24b(+)

    • 库存

      20

    • 供应商

      泽叶生物

    pET24b载体基本信息

    出品公司: ZYbscience
    别名: pET24b, pet 24b
    质粒类型: 大肠杆菌蛋白表达
    表达水平:
    克隆方法: 多克隆位点,限制性内切酶
    载体大小: 5309 bp
    5' 测序引物: T7
    5' 测序引物序列: 5'-TAATACGACTCACTATAGGG-3'
    载体标签: N-T7, C-His
    载体抗性: Kanamycin
    备注:
    Same as pET21a,b,c,d(+) but kanamycin resistance; a,b,c,d vary by MCS; The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
    产品目录号: ZY69750-3
    稳定性: 瞬时表达 Transient
    组成型: 组成型 Constitutive
    病毒/非病毒: 非病毒

    pET24b载体质粒图谱和多克隆位点信息

    pet24b载体图谱

    pet24b多克隆位点

    The maps for pET-24b(+), pET-24c(+) and pET-24d(+) are the same as pET-24a(+) (shown) with the following exceptions: pET-24b(+) is a 5309bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-24c(+) is a 5308bp plasmid; subtract 2bp from each site beyond BamH I at 198. pET-24d(+) is a 5307bp plasmid; the BamH I site is in the same reading frame as in pET- 24c(+). An Nco I site is substituted for the Nde I site with a net 1bp deletion at position 238 of pET-24c(+). As a result, Nco I cuts pET24d(+) at 234, and Nhe I cuts at 229. For the rest of the sites, subtract 3bp from each site beyond position 239 in pET-24a(+). Nde I does not cut pET-24d(+). Note also that Sty I is not unique in pET-24d(+).

    Feature Name Start End
    T7_terminator 129 1
    T7_Terminal_primer 69 87
    T7_leader 238 206
    Xpress_fwd_primer 239 221
    T7_transl_en_RBS 263 247
    lacO 308 281
    T7_promoter 326 308
    tet (300 - 563) 358 621
    pBRrevBam_primer 429 410
    lacI 704 1795
    tet (576 - 851) 1854 2129
    ROP 2604 2795
    pGEX_3_primer 2811 2789
    pBR322_origin 3829 3210
    KanR2 3935 4750
    ORF Start End
    ORF frame 1 463 963
    ORF frame 2 836 1795
    ORF frame 2 3935 4750
    Enzyme Name Cut
    XhoI 158
    NotI 166
    EagI 166
    HindIII 173
    SalI 179
    SacI 190
    EcoRI 192
    BamHI 198
    NheI 230
    NdeI 237
    XbaI 275
    BglII 341
    BclI 1077
    ApaI 1274
    EcoRV 1513
    HpaI 1569
    FspI 2145
    NruI 4023
    ClaI 4057
    XmaI 4238
    SmaI 4240

    pET24b载体简介

    The pET-24a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs. ampicillin resistance). Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, singlestranded sequencing should be performed using the T7 terminator primer.

    pET24b载体序列

    LOCUS pET-24 b (+) 5309 bp DNA circular SYN
    DEFINITION pET-24 b (+)
    ACCESSION
    KEYWORDS
    SOURCE
    ORGANISM other sequences; artificial sequences; vectors.
    COMMENT This file is created by Vector NTI
    http://www.biofeng.com/
    COMMENT ORIGDB|GenBank
    COMMENT VNTAUTHORNAME|biofeng.com|
    COMMENT VNTOAUTHORAD1|Novagen Inc.|
    FEATURES Location/Qualifiers
    source 1..5309
    /organism="pET-24 b (+)"
    /mol_type="other DNA"
    terminator complement(1..129)
    /label="T7_terminator"
    misc_feature 69..87
    /label="T7_Terminal_primer"
    misc_feature complement(206..238)
    /label="T7_leader"
    misc_feature complement(221..239)
    /label="Xpress_fwd_primer"
    misc_feature complement(247..263)
    /label="T7_transl_en_RBS"
    misc_feature complement(281..308)
    /label="lacO"
    promoter complement(308..326)
    /label="T7_promoter"
    gene 358..621
    /label="tet (300 - 563)"
    /gene="tet (300 - 563)"
    misc_feature complement(410..429)
    /label="pBRrevBam_primer"
    CDS 463..963
    /label="ORF frame 1"
    /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
    LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
    RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
    ANCRGD*"
    misc_feature 704..1795
    /label="lacI"
    /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
    LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
    RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
    ANCRGD*"
    CDS 836..1795
    /label="ORF frame 2"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    gene 1854..2129
    /label="tet (576 - 851)"
    /gene="tet (576 - 851)"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    misc_feature 2604..2795
    /label="ROP"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    misc_feature complement(2789..2811)
    /label="pGEX_3_primer"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    rep_origin complement(3210..3829)
    /label="pBR322_origin"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    gene 3935..4750
    /label="KanR2"
    /gene="KanR2"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    CDS 3935..4750
    /label="ORF frame 2"
    /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
    GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
    GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
    VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
    RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
    ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
    61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
    121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
    181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat
    241 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
    301 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc
    361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
    421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
    481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
    541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
    601 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt
    661 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
    721 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
    781 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
    841 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
    901 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
    961 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
    1021 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
    1081 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
    1141 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
    1201 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
    1261 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
    1321 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
    1381 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
    1441 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
    1501 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
    1561 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
    1621 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
    1681 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    1741 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    1801 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga
    1861 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
    1921 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
    1981 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
    2041 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
    2101 gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc
    2161 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
    2221 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
    2281 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
    2341 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
    2401 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
    2461 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
    2521 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
    2581 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
    2641 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
    2701 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
    2761 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    2821 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    2881 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
    2941 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat gcggtgtgaa
    3001 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
    3061 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    3121 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    3181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    3241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    3301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    3361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    3421 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    3481 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    3541 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    3601 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    3661 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    3721 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    3781 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    3841 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa caataaaact
    3901 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
    3961 ttgctctagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
    4021 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
    4081 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
    4141 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
    4201 tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt
    4261 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
    4321 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
    4381 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
    4441 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
    4501 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
    4561 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
    4621 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
    4681 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
    4741 gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag aaaaataaac
    4801 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata
    4861 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
    4921 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
    4981 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
    5041 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
    5101 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
    5161 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
    5221 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
    5281 cgccgctaca gggcgcgtcc cattcgcca
    //

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