| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pMal-c4X, pMalc4X, pMal c4X |
| 质粒类型: | 大肠杆菌蛋白表达载体 |
| 表达水平: | 高 |
| 启动子: | Tac |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6645 bp |
| 5' 测序引物及序列: | MalE引物: 5'-GGTCGTCAGACTGTCGATGAAGCC-3'; MBP-F: 5'-gatgaagccctgaaagacgcgcag-3' |
| 3' 测序引物及序列: | pBad-R: 5'-gatttaatctgtatcagg-3'; M13-F: 5'-TGTAAAACGACGGCCAGT-3' |
| 载体标签: | N-MBP, N-Factor Xa |
| 载体抗性: | Ampicillin 氨苄 |
| 备注: | 融合表达麦芽糖结合蛋白MBP，蛋白定位于细胞周质。 |
| 产品目录号: | ZY8000S |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 诱导表达 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pMal-c4X
- 库存：
20
- 供应商：
泽叶生物
pMalc4X载体基本信息
pMalc4X载体质粒图谱和多克隆位点信息
pMalc4X载体简介
pMalc4X载体序列
LOCUS pMAL-c4X 6645 bp DNA circular 22-OCT-2007
DEFINITION Cloning vector pMAL-c4X, complete sequence.
ACCESSION
VERSION
KEYWORDS .
SOURCE Cloning vector pMAL-c4X
ORGANISM Cloning vector pMAL-c4X
other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..6645
/organism="Cloning vector pMAL-c4X"
/mol_type="other DNA"
gene 15..1163
/gene="lacIq"
promoter 15..46
/gene="lacIq"
/label="lacIq promoter (overexpressing mutant)(clockwise)"
CDS 81..1163
/gene="lacIq"
/exception="alternative start codon"
/codon_start=1
/transl_table=11
/product="lac repressor (LacI)"
gene 1406..3307
/gene="malE expression cassette"
promoter 1406..1433
/gene="malE expression cassette"
/label="Ptac promoter (clockwise)"
CDS 1528..2916
/gene="malE expression cassette"
/label="malE sequence 1528..2628; multiple cloning site
region 2629..2734; lacZalpha sequence 2735..2916"
/codon_start=1
/product="maltose binding protein (MBP) fusion"
primer_bind 2587..2610
/label="malE primer sequence (NEB #S1237S)"
misc_feature 2695..2732
/gene="malE expression cassette"
/label="multiple cloning site (EcoRI-HindIII)"
terminator 3104..3307
/gene="malE expression cassette"
/label="rrnB T1T2 transcription terminators"
gene 3417..4277
/gene="bla"
CDS 3417..4277
/gene="bla"
/label="ampR (confers resistance to ampicillin)"
/codon_start=1
/product="beta-lactamase"
sig_peptide 3417..3485
/gene="bla"
/label="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein"
rep_origin complement(4319..4832)
/label="M13 origin of replication (+ -)"
rep_origin 4943..5531
/label="pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)"
gene complement(5961..6152)
/gene="rop"
CDS complement(5961..6152)
/gene="rop"
/exception="alternative start codon"
/codon_start=1
/transl_table=11
/product="ROP protein"
BASE COUNT 1694 a 1669 c 1769 g 1513 t
ORIGIN
1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg
1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
2461 ttggtgaaag atccgcggat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat ttcagaattc
2701 ggatcctcta gagtcgacct gcaggcaagc ttggcactgg ccgtcgtttt acaacgtcgt
2761 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
2821 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
2881 aatggcgaat ggcagcttgg ctgttttggc ggatgagata agattttcag cctgatacag
2941 attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg
3001 gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt
3061 gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
3121 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
3181 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
3241 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
3301 cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc
3361 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
3421 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
3481 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
3541 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
3601 aacgtttccc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
3661 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
3721 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
3781 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
3841 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
3901 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
3961 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
4021 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
4081 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
4141 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
4201 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
4261 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttac
4321 cccggttgat aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat
4381 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
4441 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
4501 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
4561 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc
4621 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
4681 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
4741 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
4801 cgccgcgctt aatgcgccgc tacagggcgc gtaaaaggat ctaggtgaag atcctttttg
4861 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
4921 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
4981 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
5041 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
5101 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
5161 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
5221 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
5281 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
5341 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
5401 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
5461 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
5521 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
5581 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
5641 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
5701 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
5761 accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
5821 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
5881 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
5941 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
6001 ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac agatgtctgc ctgttcatcc
6061 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
6121 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
6181 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
6241 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
6301 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
6361 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
6421 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
6481 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
6541 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
6601 acgatcatgc gcacccgtgg ccaggaccca acgctgcccg aaatt
//
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文献和实验相关实验
的工业化前景. IMPACT-TWIN系统包括两个大肠杆菌表达载体――pTWIN1,pTWIN2.之所以叫做Twin,原因在于pTWIN的设计在多克隆位点两端都各有一段intein和CBD,排列如下：几丁质结合蛋白域―intein1―MCS―Intein2―几丁质结合蛋白.这个巧妙的设计有3重便利：N-端,C-端或者N-端和C-端同时融合短的内含肽序列.想做N端融合,你可以将目的基因替换MCS―Intein2―几丁质结合蛋白,如果想做C 端融合表达,就可以将目的基因替换MCS前面的几丁质
如下：几丁质结合蛋白域―intein1―MCS―Intein2―几丁质结合蛋白。这个巧妙的设计有3重便利：N-端，C-端或者N-端和C-端同时融合短的内含肽序列。想做N端融合，你可以将目的基因替换MCS―Intein2―几丁质结合蛋白，如果想做C端融合表达，就可以将目的基因替换MCS前面的几丁质结合蛋白域―intein1―MCS，不用分2个载体；而且做过克隆的人会很有体会：很多时候双酶切载体，由于2个酶切位点都多克隆位点上，没有小片断切下来，所以是否成功双酶切就不好判断，如果用Twin载体就会切下一个片断
绝版分子生物学实用技术分享: I: 20 种用于各种DNA marker 制备的PLASMID: P1: 2k+1.5k+1k+800+700+600+500+400+300+200X2+100X4 (100BP ladder) P2: 3k+1.5k+1k+900+700+500+300X2 (200BP ladder 奇数) P3: 3k+2k+1.4k+1k+800+600+4X2 (200BP ladder 偶数) (P3\P4\P5 组合, 可拓展为1KB ladder
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pMal-c4X载体
¥800
