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- 技术资料
- 抗体名:
PKC delta/PRKCD Rabbit Polyclonal Antibody 抗体
- 抗体英文名:
PKC delta/PRKCD Rabbit Polyclonal Antibody
- 靶点:
Protein kinase C delta type
- 浓度:
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- 应用范围:
IHC, WB
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb234285
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
详询
- 形态:
Lyophilized
- 亚型:
Rabbit IgG
- 免疫原:
详询
- 规格:
100 ug
类别: Antibodies
产品子类型: Primary Antibody
描述: Anti-PKC delta/PRKCD Antibody. Tested in IF, ICC, WB applications. This antibody reacts with Human.
别名: Protein kinase C delta type; 2.7.11.13; Tyrosine-protein kinase PRKCD; 2.7.10.2; nPKC-delta; Protein kinase C delta type regulatory subunit; Protein kinase C delta type catalytic subunit; Sphingosine-dependent protein kinase-1; SDK1; PRKCD
应用稀释比例: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
免疫原: E.coli-derived human PKC delta recombinant protein (Position: M1-E160). Human PKC delta shares 95% and 93% amino acid (aa) sequence identity with mouse and rat PKC delta, respctively.
交叉反应: No cross-reactivity with other proteins
分子量: 78 kDa
防腐剂: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cancer Biology, Cell Biology
UniProt ID: Q05655
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.


产品子类型: Primary Antibody
描述: Anti-PKC delta/PRKCD Antibody. Tested in IF, ICC, WB applications. This antibody reacts with Human.
别名: Protein kinase C delta type; 2.7.11.13; Tyrosine-protein kinase PRKCD; 2.7.10.2; nPKC-delta; Protein kinase C delta type regulatory subunit; Protein kinase C delta type catalytic subunit; Sphingosine-dependent protein kinase-1; SDK1; PRKCD
应用稀释比例: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
免疫原: E.coli-derived human PKC delta recombinant protein (Position: M1-E160). Human PKC delta shares 95% and 93% amino acid (aa) sequence identity with mouse and rat PKC delta, respctively.
交叉反应: No cross-reactivity with other proteins
分子量: 78 kDa
防腐剂: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cancer Biology, Cell Biology
UniProt ID: Q05655
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.

IF analysis of PKC delta using anti-PKC delta antibody. PKC delta was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-PKC delta Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-PKC delta antibody, Western blotting. All lanes: Anti PKC delta at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: SMMC Whole Cell Lysate at 40 ug. Lane 3: U87 Whole Cell Lysate at 40 ug. Lane 4: JURKAT Whole Cell Lysate at 40 ug. Lane 5: CEM Whole Cell Lysate at 40 ug. Lane 6: A549 Whole Cell Lysate at 40 ug. Predicted bind size: 77 KD. Observed bind size: 77 KD.
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PKC delta/PRKCD Rabbit Polyclonal Antibody 抗体,orb234285,Biorbyt
¥5499






