Anti-P-glycoprotein [MRK16] 抗体,orb348849,Biorbyt产品图

Anti-P-glycoprotein [MRK16] 抗体

,orb348849,Biorbyt
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  • ¥9360
  • Biorbyt已认证
  • orb348849
  • 英国
  • 2026年07月22日
  • FC, IF, IP
  • Mouse
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      Anti-P-glycoprotein [MRK16] 抗体

    • 抗体英文名:

      Anti-P-glycoprotein [MRK16]

    • 靶点:

      P-glycoprotein

    • 浓度:

      1 mg/ml

    • 应用范围:

      FC, IF, IP

    • 宿主:

      Mouse

    • 适应物种:

      Human

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb348849

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Monoclonal

    • 保存条件:

      详询

    • 亚型:

      IgG1

    • 免疫原:

      详询

    • 规格:

      0.1 mg

    类别: Antibodies

    描述: Mouse monoclonal antibody to P-glycoprotein

    克隆号: MRK16

    别名: ABCB1; Multidrug resistance protein 1; ATP-binding cassette sub-family B member 1; CD243; MDR1; PGP; PGY1

    免疫原: Drug resistant K-562 cells.

    防腐剂: PBS with 0.02% Proclin 300.

    纯度: Purified

    特异性: Recognizes a discontinuous extracellular epitope on P-glycoprotein.

    研究领域: Cancer Biology

    UniProt ID: P08183

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Flow-cytometry using the anti-P-glycoprotein antibody MRK16 HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (orb348896, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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    图标文献和实验
    相关实验
    • Flow Cytometric Measurement of Functional and Phenotypic P-Glycoprotein

      rhodamine 123, an assay for daunorubicin accumulation, and an assay to measure P-glycoprotein levels using the MRK16 antibody. Our protocols include the use of an anti-CD45 antibody for the identification of leukemic blasts. The protocols described

    • Anti-HA Immunocytology

      monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity

    • Anti-HA Immunocytology

        Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots

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