- ￥3276
- Biorbyt
- orb372324
- 英国
- 2026年07月28日
- ELISA, IHC-P, WB
- Mouse
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AJE 润色服务限时 8 折起，权威机构信任 15+ 年Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
PEDF Mouse Monoclonal Antibody 抗体
- 抗体英文名：
PEDF Mouse Monoclonal Antibody
- 靶点：
PEDF
- 浓度：
- 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
- 应用范围：
ELISA, IHC-P, WB
- 宿主：
Mouse
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb372324
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 形态：
PBS with 0.05% sodium azide
- 亚型：
IgG1
- 免疫原：
详询
- 规格：
100 ug
描述: PEDF Mouse Monoclonal Antibody is an unconjugated antibody that specifically recognizes PEDF. Generated against a Recombinant protein of human PEDF. The suitable applications for this antibody are ELISA, IHC-P and WB.
克隆号: 4F6H9
别名: anti EPC 1 antibody, anti EPC-1 antibody, anti EPC1 antibody, anti PEDF antibody, anti PIG 35 antibody, anti PIG35 antibody, anti Serpin F1 antibody, anti SERPINF 1 antibody, anti Serpinf1 antibody, anti Alpha 2 antiplasmin antibody, anti Cell proliferation-inducing gene 35 protein antibody
应用稀释比例: WB:1:1000-1:2000, IHC-P: 1:100-500
免疫原: Recombinant protein of human PEDF
分子量: 47 kDa
纯度: Purified by protein G.
研究领域: Musculoskeletal & Connective Tissue Research
UniProt ID: P36955, P97298
NCBI 参考序列: 002606
Entrez 基因 ID: 5176
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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