- ￥10062
- Biorbyt
- orb345236
- 英国
- 2026年08月05日
- ELISA, IHC, WB
- Rabbit
- Mouse
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- 文献和实验
- 技术资料
- 抗体名：
Il27 Antibody (Biotin) 抗体
- 抗体英文名：
Il27 Antibody (Biotin)
- 靶点：
Il27
- 浓度：
1.0 mg/mL
- 应用范围：
ELISA, IHC, WB
- 宿主：
Rabbit
- 适应物种：
Mouse
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb345236
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Lyophilized
- 亚型：
IgG
- 免疫原：
详询
- 规格：
100 ug
产品子类型: Primary Antibody
描述: IL-27/p28 antibody (Biotin)
别名: rabbit anti-IL-27/p28 biotin conjugated antibody, rabbit anti-interleukin 27a biotin conjugated antibody, Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30
应用稀释比例: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
应用说明: IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23.6 kDa in size corresponding to the mature mouse IL-27/p28 protein by western blotting in appropriate cell lysate or extract.
免疫原: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.
防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
纯度: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-27/p28 protein. A BLAST analysis was used to suggest cross-reactivity with IL-27A/p28 from mouse sources based on 100% homology with the immunizing sequence. Based on 90% or greater positive homology, there is a chance of cross-reactivity to rat. Cross-reactivity with IL-27 from other sources has not been determined.
研究领域: Cell Biology
UniProt ID: Q8K3I6
NCBI 参考序列: NP_663611
保存条件: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
备注: For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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