- ￥3276
- Biorbyt
- orb371930
- 英国
- 2026年07月22日
- FC, IHC-Fr
- Human, Primate
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- 文献和实验
- 技术资料
- 抗体名：
TCR gamma/delta Antibody 抗体
- 抗体英文名：
TCR gamma/delta Antibody
- 靶点：
TCR gamma/delta
- 浓度：
1 mg/ml
- 应用范围：
FC, IHC-Fr
- 适应物种：
Human, Primate
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb371930
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG1 kappa
- 免疫原：
详询
- 规格：
0.1 mg
产品子类型: Primary Antibody
描述: Mouse monoclonal antibody to TCR gamma/delta
克隆号: B1
别名: TCRG/D
应用说明: Flow cytometry: Recommended dilution: 1-4 μg/ml. The reactivity of clone B1 (anti-TCR gamma/delta) may be affected by co-staining with an anti-human CD3 mAb, which is likely to block the epitope recognized by clone B1. When using antibody B1 in a panel that includes CD3, it is recommended to apply a staining protocol in which the B1 mAb is added to the sample and incubated for approximately 15 minutes before adding the remaining antibodies. No washing is required between the staining steps.
防腐剂: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
纯化方法: Purified by protein-A affinity chromatography.
特异性: The mouse monoclonal antibody B1 (also known as B1.1) recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
Entrez 基因 ID: 35
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies
cytometry application for determining the number of peptide–MHC complexes on the surface of cells grown in tissue culture that express an endogenous protein from which the peptide is derived. The procedure requires a monoclonal antibody with the ability
板，利用 ELISA 方法测定抗体亲和力，可达到高通量检测效果。Anna Sergeeva 等（2011）在研究人类急性髓细胞样白血病的 TCR 样抗体工作中，用 HLA-A2-PR1 多肽复合物免疫小鼠，分离小鼠脾脏细胞。培养单克隆细胞，制备抗体。将 MHC-PR1 多肽复合物包被于酶联板上，进行同量化 ELISA 筛选。在 2850 个克隆中，筛选到一个（8F4）具备亲和力的抗体。推荐阅读：1. TCR Mimic Monoclonal Antibody Targets a Specific
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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