Monkey IgM Antibody 蛋白,orb346440,Biorbyt产品图
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Monkey IgM Antibody 蛋白,orb3464

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  • ¥6669
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  • 英国
  • orb346440
  • 2026年08月06日
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    • 详细信息
    • 文献和实验
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    • 保存条件

      详询

    • 保质期

      12 months from date of receipt.

    • 英文名

      Monkey IgM Antibody

    • 库存

      99

    • 供应商

      Biorbyt

    • 规格

      250 ug

    类别: Proteins

    描述: Monkey IgM Antibody

    别名: Monkey Immunoglobulin M

    浓度: 1.0 mg/mL

    形态: Liquid (sterile filtered)

    亚型: IgM

    应用: SDS-PAGE

    应用稀释比例: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized

    应用说明: Monkey IgM whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

    标记物: Unconjugated

    生物来源: Monkey

    防腐剂: Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

    纯度: Monkey IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Monkey IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Monkey Serum and anti-Monkey IgM (µ chain specific). No reaction was observed against anti-Monkey IgG F(c). Some light chain cross reactivity will occur with anti-Monkey IgG.

    来源: Monkey

    研究领域: Protein Biochemistry

    保存条件: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

    备注: For research use only.

    产品细节图片1
    Humoral immune response. Plasmas were analyzed before vaccination (week 0, pre-immune) and at weeks 1, 2, 4 and 6 post-vaccination for anti-VSV antibodies. Neutralizing antibody (NA) titer (left panel) was defined as the dilution of serum that inhibited infection of mouse EL4 cells with M51R-eGFP by 50%. IgM [p/n orb346440] (middle panel) and IgG [p/n orb2652733] (right panel) levels are expressed in ng/mL (mean ± SD, triplicate values), measured by ELISA with extrapolation from standard curves. Data for low dose (a) and high dose (b) vaccinations with M51R and M51R-F are shown. Note scale differences for low and high dose data. Statistical significance was determined by two-factor analysis of variance with cohort and time as the two factors. For all three antibody types, statistical significance of p < 0.05 (after correction for multiple comparisons) was obtained for comparisons of cohort 1 versus cohort 3 (low dose M51R vs. M51R-F), cohort 1 versus cohort 2 (low dose M51R vs. high dose M51R), and cohort 3 versus cohort 4 (low dose M51R-F vs. high dose M51R-F), but not for cohort 2 versus cohort 4 (high dose M51R vs. high dose M51R-F). The data shown represent 1 of 2 analyses with similar results performed on plasma from each animal.

    产品细节图片2
    SDS-PAGE of Monkey IgM Whole Molecule. Lane 1: Monkey IgM, Non-Reduced. Lane 2: Monkey IgM, Reduced. Load: 1.0 µg per lane. Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.

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    图标文献和实验
    该产品被引用文献
    Westcott, Marlena M. et al. Immunogenicity in African Green Monkeys of M Protein Mutant Vesicular Stomatitis Virus Vectors and Contribution of Vector-Encoded Flagellin Vaccines (Basel), 6, E16 (2018)
    相关实验
    • Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays

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    • 抗体(antibody

      。此时若再次接触抗原,可使已消失的抗体快速上升。如再次刺激机体的抗原与初次相同,则称为特异性回忆反应;若与初次反应不同,则称为非特异性回忆反应。非特异性回忆反应引起的抗体的上升是暂时性的,短时间内即很快下降。   抗体的分类 (1)按作用对象,可将其分为抗毒素、抗菌抗体、抗病素抗体和亲细胞抗体(能与细胞结合的免疫球蛋白,如1型变态反应中的lgE反应素抗体,能吸附在靶细胞膜上)。(2)按理化性质和生物学功能,可将其分为IgG、IgA、IgM、IgE、IgD五类。(3)按与抗原结合后是否出现

    • Protein A Purification of Antibody

      PBS, overnight, 2-8℃.Re-equilibrate column with Binding Buffer. Store column at 2-8℃.Determine protein concentration of Ab, aliquot, and store at -20℃.3. NoteExtinction Coefficient for IgG = 1.4 and for IgM = 1.2

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