- ￥3120
- biorbyt
- orb317579
- 英国
- 2025年12月02日
- FC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Caspase-1 p20 Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Caspase-1 p20 Rabbit Polyclonal Antibody
- 靶点：
CASP1
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb317579
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from mouse Caspase-1 p20 (181-280/402aa)
- 规格：
50 ul
产品描述：Caspase-1 p20 Rabbit Polyclonal Antibody
别名:caspase-1 isoform alpha p20 subunit; CASP1; ICE; IL1BC; Caspase-1 subunit p20; CASP 1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BCE; Interleukin 1 beta convertase; Interleukin 1 beta convertase precursor; Interleukin 1 beta converting enzyme; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; CASP1_HUMAN; CASP1_MOUSE.
免疫原：KLH conjugated synthetic peptide derived from mouse Caspase-1 p20 (181-280/402aa)
预测反应性:Human, Rat
分子量:20/46 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
防腐剂: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P29452
Note:For research use only.
别名:caspase-1 isoform alpha p20 subunit; CASP1; ICE; IL1BC; Caspase-1 subunit p20; CASP 1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BCE; Interleukin 1 beta convertase; Interleukin 1 beta convertase precursor; Interleukin 1 beta converting enzyme; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; CASP1_HUMAN; CASP1_MOUSE.
免疫原：KLH conjugated synthetic peptide derived from mouse Caspase-1 p20 (181-280/402aa)
预测反应性:Human, Rat
分子量:20/46 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
防腐剂: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P29452
Note:For research use only.
Blank control (blue line): MCF7 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (orb317579), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC.
Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (orb317579), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: large intestine (rat) Lysate at 40 ug, Primary: Anti-Caspase-1 p20 (orb317579) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20/46kD, Observed band size: 46kD.
Sample: lung (rat) Lysate at 40 ug, Primary: Anti-Caspase-1 p20 (orb317579) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20/46kD, Observed band size: 46kD.
Tissue/Cell: Mouse spleen tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Caspase-1 p20 Polyclonal Antibody, Unconjugated (orb317579) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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文献和实验相关实验
-277): sc-7148 SOURCE Caspase-3 (H-277) is a rabbit polyclonal antibody raised against a recombinant protein corresponding to amino acids 1-277 representing the full length precursor form of caspase-3 of human origin. SPECIFICITY Caspase-3 (H-277
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Caspase-1 p20 Rabbit Polyclonal Antibody抗体，orb317579，biorbyt
￥3120
