- ￥5967
- Biorbyt
- orb343776
- 英国
- 2026年08月05日
- ELISA, FC, SDS-PAGE
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- 文献和实验
- 技术资料
- 抗体名：
Mouse IgG1 isotype Control Antibody 抗体
- 抗体英文名：
Mouse IgG1 isotype Control Antibody
- 浓度：
1.0 mg/mL
- 应用范围：
ELISA, FC, SDS-PAGE
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb343776
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 保存条件：
详询
- 形态：
Liquid (sterile filtered)
- 亚型：
IgG1
- 免疫原：
详询
- 规格：
1 mg
描述: Mouse IgG1 isotype Control Antibody
克隆号: MG1
别名: Mouse IgG1 isotype, Mouse IgG1 subclass isotype, Mouse IgG1 Control, Mouse isotype Control
应用稀释比例: FC: 1:1000-1:5000
应用说明: Mouse IgG1 isotype control has been tested by ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
生物来源: Mouse
防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
纯度: Mouse IgG1 Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG1 and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
来源: Mouse
研究领域: Protein Biochemistry
保存条件: Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
备注: For research use only.
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
The history of isotype selection for therapeutic antibodies clearly demonstrates that solid understanding of the biologic effects mediated by immunoglobulin (Ig) subclasses IgG1, IgG2, IgG3, and IgG4 and their variants is a prerequisite
Isotype Determination of Monoclonal Antibodies by Immunodiffusion
antibodies are allowed to diffuse toward anti-mouse isotype specific antisera in an agarose gel. In a positive reaction, a precipitin line will develop in the agarose between two wells. A monoclonal antibody should show only one heavy chain and one light
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