- ￥3120
- biorbyt
- orb182470
- 英国
- 2025年12月09日
- FC, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Porcine
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- 详细信息
- 技术资料
- 抗体名：
CD4 Rabbit Polyclonal Antibody抗体
- 抗体英文名：
CD4 Rabbit Polyclonal Antibody
- 靶点：
CD4
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Porcine
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb182470
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human CD4 (385-457/457aa)
- 规格：
50 ul
产品描述：CD4 Rabbit Polyclonal Antibody
别名:CD4(L3T4); CD4 antigen(p55); CD4 Antigen; CD4 molecule; CD4 Receptor; CD4+Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3; T cell surface antigen T4/Leu3; T Cell Surface Glycoprotein CD4; W3/25; W3/25 antigen; T-cell surface glycoprotein CD4 isoform 1 precursor; CD4_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human CD4 (385-457/457aa)
预测反应性:Bovine, Canine, Guinea pig, Rat, Sheep
分子量:48 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P01730
Note:For research use only.
别名:CD4(L3T4); CD4 antigen(p55); CD4 Antigen; CD4 molecule; CD4 Receptor; CD4+Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3; T cell surface antigen T4/Leu3; T Cell Surface Glycoprotein CD4; W3/25; W3/25 antigen; T-cell surface glycoprotein CD4 isoform 1 precursor; CD4_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human CD4 (385-457/457aa)
预测反应性:Bovine, Canine, Guinea pig, Rat, Sheep
分子量:48 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P01730
Note:For research use only.
Blank control (blue line): HL60 (fixed with 2% Fixative (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD4 antibody (orb182470), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.
Fixative-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (orb182470) at 1:4000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (orb182470) at 1:4000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody at 1:300 for one hour at room temperature.
Sample: Lane 1: U937 (Human) Cell Lysate at 30 ug, Lane 2: HL-60 (Human) Cell Lysate at 30 ug, Lane 3: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-CD4 (orb182470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.
Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-CD4 (orb182470) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 55kD.
Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CD4 (orb182470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 55 kD.
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CD4 Rabbit Polyclonal Antibody抗体，orb182470，biorbyt
￥3120
