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CD4 Rabbit Polyclonal Antibody

抗体,orb182470,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb182470
  • 英国
  • 2025年12月09日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Porcine
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      CD4 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      CD4 Rabbit Polyclonal Antibody

    • 靶点

      CD4

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Porcine

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb182470

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human CD4 (385-457/457aa)

    • 规格

      50 ul

    产品描述:CD4 Rabbit Polyclonal Antibody

    别名:CD4(L3T4); CD4 antigen(p55); CD4 Antigen; CD4 molecule; CD4 Receptor; CD4+Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3; T cell surface antigen T4/Leu3; T Cell Surface Glycoprotein CD4; W3/25; W3/25 antigen; T-cell surface glycoprotein CD4 isoform 1 precursor; CD4_HUMAN.

    免疫原:KLH conjugated synthetic peptide derived from human CD4 (385-457/457aa)

    预测反应性:Bovine, Canine, Guinea pig, Rat, Sheep

    分子量:48 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P01730

    Note:For research use only.

    产品细节图片1
    Blank control (blue line): HL60 (fixed with 2% Fixative (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD4 antibody (orb182470), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

    产品细节图片2
    Fixative-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (orb182470) at 1:4000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (orb182470) at 1:4000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody at 1:300 for one hour at room temperature.

    产品细节图片5
    Sample: Lane 1: U937 (Human) Cell Lysate at 30 ug, Lane 2: HL-60 (Human) Cell Lysate at 30 ug, Lane 3: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-CD4 (orb182470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.

    产品细节图片6
    Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-CD4 (orb182470) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 55kD.

    产品细节图片7
    Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CD4 (orb182470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 55 kD.

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    图标文献和实验
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    • Polyclonal Antibody Production

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