- ￥2691
- Biorbyt
- orb222725
- 英国
- 2026年07月22日
- FC
- Mouse
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- 文献和实验
- 技术资料
- 抗体名：
CD90.2 Antibody (PE) 抗体
- 抗体英文名：
CD90.2 Antibody (PE)
- 靶点：
CD90.2
- 浓度：
0.1 mg/ml
- 应用范围：
FC
- 适应物种：
Mouse
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb222725
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
PE
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG3
- 免疫原：
详询
- 规格：
0.1 mg
产品子类型: Primary Antibody
描述: Mouse monoclonal antibody against CD90.2 conjugated to PE
克隆号: 1aG4/C5
别名: Thy-1.2
应用说明: Flow cytometry: Recommended dilution: 1-2 μg/ml.
免疫原: thymocytes from C3H/Di mice (Thy-1.2 positive)
防腐剂: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
纯化方法: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
特异性: The mouse monoclonal antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90); it does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI anchored glycoprotein (immunoglobulin superfamily); in mouse it is abundant on thymocytes and peripheral T lymphocytes.
研究领域: Immunology & Inflammation
UniProt ID: P01831
Entrez 基因 ID: 21838
保存条件: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
备注: For research use only.
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文献和实验Western Blotting with Horseradish Pe
of proteins from 10% or 13% gels to PVDF membranes semi-dry transfer can also be used. Transfer proteins to PVDF membrane at 1.2 mAmp/cm2 for 1 hour and 45 minutes in transfer buffer (25mM Tris, 190mM glycine, 20% MeOH). Optional If blots are not to be used
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
TGT 3' Length: 21-mer 3' 948 Tm: 65.7 °C (salt 1000.0 mM; oligo 100.0 pM) deltaG (25 °C): -40.0 kcal/mol Degeneracy: 1 P.E.#: 298/403 1/E: 5.16 nmol/A260 33.2 μg/A260 以上是我用oligo得到的引物，引物二聚体，发夹结构等都没有问题，可是总得不到我想要的带。从结果上看Lower Primer 的 P.E
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