- ￥6600
- Agrisera
- AS09 466a
- 美国
- 2025年07月14日
- ELISA (ELISA), western blot (WB)
- 兔
- 植物
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
兔抗拟南芥液泡H+-ATPase a亚基多克隆抗体
- 抗体英文名：
V-ATPase,a|vacuolar H+ ATPase,subunit a
- 浓度：
见说明书
- 应用范围：
ELISA (ELISA), western blot (WB)
- 宿主：
兔
- 适应物种：
植物
- 标记物：
无
- 克隆性：
多抗/单行
- 保存条件：
低温
- 亚型：
IgG
- 规格：
100 µl
供应商：上海经科化学科技有限公司
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V-ATPase,a|vacuolar H+ ATPase,subunit a介绍：
货 号：AS09 466
中文名称：兔抗拟南芥液泡H+-ATPase a亚基多克隆抗体
英文名称：V-ATPase,a|vacuolar H+ ATPase,subunit a
应用：ELISA (ELISA), western blot (WB)
规格：100 µl
价格：6600元
V-ATPase,a|vacuolar H+ ATPase,subunit a简介：
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| APPLICATION INFORMATION | ||
| recommended dilution | 1: 8000 (ELISA), 1: 2000 with standard ECL (WB) |
|
| expected | apparent MW | 93 | 100 kDa (Arabidopsis thaliana) |
|
| confirmed reactivity | Arabidopsis thaliana, Cucumis sativus |
|
| predicted reactivity | dicots including: Ricinus communis, Vitis vinifera, monocots including: Oryza sativa, trees.: Populus balsamifera, moss: Physcomitrella patens, algae: Chlamydomonas reainhardtii |
|
| not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
|
| additional information | Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC. Manufactured by Operon Biotechnologies. |
|
| selected references | Migocka et al. (2013). NO3 (-)/H(+) Antiport in the Tonoplast of Cucumber Root Cells Is Stimulated by Nitrate Supply: Evidence for a Reversible Nitrate-Induced Phosphorylation of Vacuolar NO3 (-)/H(+) Antiport.PLoS One. 2013 Sep 11;8(9):e73972. doi: 10.1371/journal.pone.0073972. Fumiyoshi et al. (2005). Novel type aquaporin SIPs, are mainly localized the ER membrane and show cell-specific expression in Arabidopsis thaliana. FEBS Lett. 579: 5814-58200. Yoshihiro et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 45: 1749-1758. |
|
| application example
1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powderin TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AJ011604 | nitrate transporter antibodies (AS09 466, 1:2000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. |
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文献和实验Steady State ATPase Assays Coupled Enzyme System
, in Tris-HCl. Avoid using phosphate buffers for ATPase assays. 2. The coupled enzyme ATPase assay is based on the conversion of phosphoenolpyruvate (PEP) to pyruvate by pyruvate kinase (PK) coupled to the conversion
能够水解ATP,并利用ATP水解释放出的能量驱动物质跨膜运输的运输蛋白称为运输ATPase, 由于它们能够进行逆浓度梯度运输, 所以有称为泵。共有四种类型的运输ATPase: ① P型离子泵(P-type ion pump),或称P型ATPase 。此类运输泵运输时需要磷酸化(P是phosphorylation的缩写),包括Na -K 泵、Ca2 离子泵。 ② V型泵(V-type pump),或称V型ATPase,主要位于小泡的膜上( V代表
Q：Annexin V 凋亡检测试剂盒的原理是什么？ A：Annexin V 是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸 PS 有高度亲和力，可通过细胞外侧暴露的 PS 与凋亡早期细胞胞膜结合，将 Annexin V 标记荧光染料如 FITC 利用流式细胞仪或荧光显微镜可检测细胞凋亡。 碘化丙啶(Propidium Iodide, PI)或 7-AAD 可与双链 DNA 特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，核染料 PI 或 7-AAD
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