- 询价
- Signalway Antibody
- 45403
- USA
- 2025年07月09日
- WB
- Rabbit
- Hu, Ms, Rt
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
V-ATPase D1 Antibody
- 靶点：
V-ATPase D1
- 浓度：
1.0mg/ml
- 应用范围：
WB
- 宿主：
Rabbit
- 适应物种：
Hu, Ms, Rt
- 克隆性：
Polyclonal
- 保存条件：
Store at -20℃
- 形态：
liquid
- 免疫原：
A synthesized peptide derived from human V-ATPase D1
Accession No.Swiss-Prot: P61421;NCBI Gene ID:9114
Alternative Names：ATP6V0D1, 32 kDa accessory protein, p39, V-ATPase, subunit D, VPATPD, V-ATPase subunit d 1, VATX, VMA6, ATP6D, ATP6DV, V-ATPase AC39 subunit, V-ATPase d subunit
产品详细信息请点击：V-ATPase D1 Antibody
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文献和实验To engineer or manipulate antibody or Fv-based molecules, isolation of the V region of the antibodies is necessary. A number of strategies can be adopted for amplification; one such approach is to use subgroup-specific oligonucleotides
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E Procedure Prepare the cell type of interest as a single cell suspension and wash once with cold PBS
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