- ￥5565
- Agrisera
- AS09 577Aa
- 美国
- 2025年07月13日
- western blot, (WB)
- 兔
- 植物
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
兔抗拟南芥液泡膜H+ATPase ε亚基多克隆抗体
- 抗体英文名：
V-ATPase|epsilon subunit of tonoplast H+ATPase (affinty purified)
- 浓度：
见说明书
- 应用范围：
western blot, (WB)
- 宿主：
兔
- 适应物种：
植物
- 标记物：
无
- 克隆性：
多抗/单行
- 保存条件：
低温
- 亚型：
IgG
- 规格：
200 µg
供应商：上海经科化学科技有限公司
服务热线：400-0199-638
QQ:472482400(上海经科）
微信号：shjkchem
活动：消费积分可换充值卡！
V-ATPase|epsilon subunit of tonoplast H+ATPase (affinty purified)介绍：
货 号：AS09 577A
中文名称：兔抗拟南芥液泡膜H+ATPase ε亚基多克隆抗体
英文名称：V-ATPase|epsilon subunit of tonoplast H+ATPase (affinty purified)
应用：western blot, (WB)
规格：200 µg
价格：5565元
V-ATPase|epsilon subunit of tonoplast H+ATPase (affinty purified)简介：
|
|||||||||||||||||||||||||||||||||||||||||
| APPLICATION INFORMATION | ||
| recommended dilution | 1 : 1 000 with alkaline phosphatase or 1: 3000 with ECL (WB) |
|
| expected | apparent MW | 26 | 31 kDa (Arabidopsis thaliana) |
|
| confirmed reactivity | dicots including: Arabidopsis thaliana, Medicago truncatula, Nicotiana tabacum, Solanum lycopersicum, monocots including: Avena strigosa |
|
| predicted reactivity | Chlamydomonas reinhardtii, Hordeum vulgare, Malus domestica, Mesembryanthemum sp., Oryza sativa, Petunia sp.,Phaseolus sp. ,Physcomitrella patens, Pteris vittata (fern), Ricinus communis,Thellungiella sp., Zea mays, algae, Vitis vinifera bull frog, chicken, bovine, Drosophila melanogaster,human, mouse, rat |
|
| not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
|
| additional information | V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample. 2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background. |
|
| selected references | to be added when available. Antibody released in November 2009 |
|
| application example 6 µg of total SDS-extracted protein from Avena strigosa roots (R) and leaves (L) , were separated on NuPage LDS-PAGE 4-12% gradient acrylamide gel (Invitrogen) and blotted 1h to nitrocellulose. Filters were blocked 1h with 5% low-fat milk powder in TBS and probed with anti-V-ATPase antibodies (AS09 577 , 1:2000, 1h) and secondary anti-goat (1:5000, 1 h) antibody (Alexa 647) in TBS containing 5% low fat milk powder. Antibody incubations were followed by washings in TBS-T (containing 0.05% Tween-20, 0.1% Triton X-100) . All steps were performed at RT with agitation. Blots were scanned with a Typhoon scanner. Courtesy Dr. Sam Mugford (JIC)
|
 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Steady State ATPase Assays Coupled Enzyme System
, in Tris-HCl. Avoid using phosphate buffers for ATPase assays. 2. The coupled enzyme ATPase assay is based on the conversion of phosphoenolpyruvate (PEP) to pyruvate by pyruvate kinase (PK) coupled to the conversion
能够水解ATP,并利用ATP水解释放出的能量驱动物质跨膜运输的运输蛋白称为运输ATPase, 由于它们能够进行逆浓度梯度运输, 所以有称为泵。共有四种类型的运输ATPase: ① P型离子泵(P-type ion pump),或称P型ATPase 。此类运输泵运输时需要磷酸化(P是phosphorylation的缩写),包括Na -K 泵、Ca2 离子泵。 ② V型泵(V-type pump),或称V型ATPase,主要位于小泡的膜上( V代表
Q：Annexin V 凋亡检测试剂盒的原理是什么？ A：Annexin V 是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸 PS 有高度亲和力，可通过细胞外侧暴露的 PS 与凋亡早期细胞胞膜结合，将 Annexin V 标记荧光染料如 FITC 利用流式细胞仪或荧光显微镜可检测细胞凋亡。 碘化丙啶(Propidium Iodide, PI)或 7-AAD 可与双链 DNA 特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，核染料 PI 或 7-AAD
技术资料暂无技术资料 索取技术资料
