Prevail™ Select C18, 5µm, 150

T7Select 噬菌体展示系统

、包装、表达和亲和掏洗的高质量试剂盒以及详细的优化操作步骤指导。大通量亲和淘洗T7极其稳定，在亲和掏洗时可以采用各种试剂以将噬菌体从固相结合物上释放下来，例如1% SDS，5 M NaCl，4 M尿素，2 M盐酸胍，10 mM EDTA，100 mM DTT，pH 4-10。高代表性文库构建与展示采用高效包装提取物和T7克隆试剂盒能轻易地构建大的展示文库。有高、中、低拷贝表达供选择用户可以根据表达和亲和掏洗(蛋白结合)的具体要求方便地选择相应的高拷贝或低拷贝表达载体: T7Select系统提供多种

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

at �80°C. (14) Aliquot 100 µl of the supernatants into 1.5 ml microfuge tubes, and add 900 µl of 150 mM NaCl ChIP Dilution buffer/1x proteinase inhibitors. This dilution decreases the SDS concentration in chromatin samples

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded