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pET-29a(+)载体

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  • ZY69871-3
  • 2025年07月12日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      pET-29a(+)

    • 库存：

      20

    • 供应商：

      泽叶生物

    pET29a载体基本信息

    出品公司: EMD Biosciences (Novagen)
    别名: pET29a, pet 29a
    质粒类型: 大肠杆菌蛋白表达
    表达水平: 高
    克隆方法: 多克隆位点，限制性内切酶
    载体大小: 5371 bp
    5' 测序引物: T7
    5' 测序引物序列: 5'-TAATACGACTCACTATAGGG-3'
    载体标签: N-S, N-Thrombin, C-His
    载体抗性: Kanamycin
    表达宿主菌: BL21(DE3) 、BLR(DE3)、BL21(DE3)pLysS
    备注:
    N term thrombin cleavage site; a,b,c vary by MCS;
    产品目录号: ZY69871-3
    稳定性: 瞬时表达 Transient
    组成型: 组成型 Constitutive
    病毒/非病毒: 非病毒

    pET29a载体质粒图谱和多克隆位点信息

    pet29a载体图谱
    pet29a多克隆位点

    Feature Name Start End
    T7_terminator 129 1
    T7_Terminal_primer 69 87
    lacI 218 1857
    T7_transl_en_RBS 321 305
    lacO 366 339
    T7_promoter 384 366
    tet (300 - 563) 420 683
    pBRrevBam_primer 491 472
    tet (576 - 851) 1916 2191
    ROP 2666 2857
    pGEX_3_primer 2873 2851
    pBR322_origin 3891 3272
    KanR2 3997 4812
    ORF Start End
    ORF frame 3 297 1025
    ORF frame 1 898 1857
    ORF frame 1 3997 4812
    Enzyme Name Cut
    XhoI 158
    NotI 166
    EagI 166
    HindIII 173
    SalI 179
    SacI 190
    EcoRI 192
    BamHI 198
    EcoRV 206
    NcoI 212
    KpnI 238
    BglII 241
    BstBI 268
    NdeI 295
    XbaI 333
    BclI 1139
    ApaI 1336
    HpaI 1631
    FspI 2207
    NruI 4085
    SmaI 4302
    XmaI 4300

    pET29a载体简介

    The pET-29a-c(+) vectors carry an N-terminal S•Tag™/thrombin configuration plus an optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No.69337-3).

    pET29a载体序列

    LOCUS pET-29 a (+) 5371 bp DNA Circular SYN
    DEFINITION pET-29 a (+)
    ACCESSION
    KEYWORDS
    SOURCE
    ORGANISM other sequences; artificial sequences; vectors.
    COMMENT This file is created by Vector NTI
    http://www.biofeng.com/
    COMMENT ORIGDB|GenBank
    COMMENT VNTAUTHORNAME|biofeng.com|
    COMMENT VNTOAUTHORAD1|Novagen Inc.|
    FEATURES Location/Qualifiers
    source 1..5371
    /organism="pET-29 a (+)"
    /mol_type="other DNA"
    terminator complement(1..129)
    /label="T7_terminator"
    misc_feature 69..87
    /label="T7_Terminal_primer"
    misc_feature 218..1857
    /label="lacI"
    CDS 297..1025
    /label="ORF frame 3"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature complement(305..321)
    /label="T7_transl_en_RBS"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature complement(339..366)
    /label="lacO"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    promoter complement(366..384)
    /label="T7_promoter"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    gene 420..683
    /label="tet (300 - 563)"
    /gene="tet (300 - 563)"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature complement(472..491)
    /label="pBRrevBam_primer"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    CDS 898..1857
    /label="ORF frame 1"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    gene 1916..2191
    /label="tet (576 - 851)"
    /gene="tet (576 - 851)"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    misc_feature 2666..2857
    /label="ROP"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    misc_feature complement(2851..2873)
    /label="pGEX_3_primer"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    rep_origin complement(3272..3891)
    /label="pBR322_origin"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    gene 3997..4812
    /label="KanR2"
    /gene="KanR2"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    CDS 3997..4812
    /label="ORF frame 1"
    /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
    GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
    GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
    VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
    RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
    ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
    61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
    121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
    181 cgacggagct cgaattcgga tccgatatca gccatggaac cgcgtggcac cagggtaccc
    241 agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatatgt
    301 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca
    361 attcccctat agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctctacg
    421 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
    481 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
    541 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
    601 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
    661 tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
    721 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
    781 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
    841 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
    901 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
    961 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
    1021 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
    1081 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
    1141 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
    1201 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
    1261 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
    1321 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
    1381 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
    1441 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
    1501 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
    1561 cgcgttggtg cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
    1621 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
    1681 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
    1741 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    1801 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    1861 caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt
    1921 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
    1981 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
    2041 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
    2101 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
    2161 cggcgagaag caggccatta tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct
    2221 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc
    2281 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
    2341 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc
    2401 ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc
    2461 tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg
    2521 catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc
    2581 agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag
    2641 aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc
    2701 ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga
    2761 tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg
    2821 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    2881 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    2941 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
    3001 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg
    3061 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
    3121 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    3181 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    3241 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    3301 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    3361 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    3421 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    3481 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    3541 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    3601 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    3661 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    3721 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    3781 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    3841 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    3901 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aacaataaaa
    3961 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
    4021 tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
    4081 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat
    4141 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
    4201 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
    4261 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag
    4321 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
    4381 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
    4441 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
    4501 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
    4561 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
    4621 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
    4681 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
    4741 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
    4801 gagtttttct aagaattaat tcatgagcgg atacatattt gaatgtattt agaaaaataa
    4861 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa
    4921 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
    4981 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
    5041 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
    5101 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
    5161 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
    5221 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
    5281 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
    5341 tgcgccgcta cagggcgcgt cccattcgcc a

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