- ￥1534.68
- Lucigen
- http://lucigen.com/store/docs/manuals/MA018_Ecloni_EXPRESS_Electrocompetent_Cells.pdf
- 60300-1
- 2026年04月08日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80º C
- 英文名：
E. cloni® EXPRESS BL21(DE3) Electrocompetent Cells
- 库存：
大量
- 供应商：
中北林格
- 规格：
12 rxns
E. cloni® EXPRESS BL21(DE3) Competent Cells
产品说明书
- Best value for routine protein expression... available as Electrocompetent and Chemically Competent Cells.
Lucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells (Figure 1), which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (> 5 × 109 cfu/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment (Figure 2).
_graph.png) |
| Figure 1. Transformation efficiency comparison of commercially available BL21(DE3) competent cells.
|
| |
| Figure 2. Comparison of cloning and protein expression procedures. |
E. cloni EXPRESS BL21(DE3) strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.
NOTE: For best results in expressing proteins that may be toxic, we recommend using OverExpress Cells. E. cloniEXPRESS cells are also available as chemically competent cells. With a transformation efficiency of >1 × 107 cfu/µg and very economical prices, EXPRESS Chemically Competent Cells offer superior value for everyday protein expression work.
-
Ordering
-
Specifications
-
Manuals
-
Resources
Each E. cloni EXPRESS Kit contains: the indicated E. cloni EXPRESS Electrocompetent or Chemically Competent Cells in DUO packaging (2 transformations per tube), Expression Recovery Medium (lactose minus), pUC19 Positive Control Plasmid, and complete protocols.
欢迎您咨询订购北京中北林格科技发展有限公司代理的Lucigen全系列产品，
Lucigen中国区一级代理北京中北林格科技发展有限公司
010-88891086 www.bjzblg.com
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Stable Expression Clones and Auto-Induction for Protein Production in E. coli
, and burdensome inoculation with fresh colonies, sometimes employed in hopes of assuring high yields, is entirely unnecessary. These media were developed for the T7 expression system using pET vectors in BL21(DE3) but are suitable or adaptable for other inducible
Transformation of plasmid DNA to competent E. coli cells Transformation is the process of getting the recombinant vector from a reaction mixture or vector solution into E. coli cells. To enable the cells to take up circular vector DNA
; as well as recombinant trypsin-like proteolytic enzymes produced from bacterial fermentation (i.e., TrypLE® Express commercially available from Gibco, Gaithersburg, MD, USA). However, it must be noted that enzymatic cell dissociation inevitably results
技术资料暂无技术资料 索取技术资料
