- ￥1958.04
- Lucigen
- http://lucigen.com/store/docs/manuals/MA003-CloneDirect-Rapid-Ligation-Kit.pdf
- 40020-4
- 2026年01月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20º C
- 英文名：
CloneDirect™ Rapid Ligation Kit
- 库存：
大量
- 供应商：
中北林格
- 规格：
48 rxns
CloneDirect™ Rapid Ligation Kit
- Ligation reaction is transformation-ready in minutes
- No DNA cleanup step required
- Highly reliable and reproducible results
Quicken cloning
The CloneDirect Rapid Ligation Kit is a simple, rapid, highly efficient alternative to standard ligation and transformation protocols. This kit eliminates the requirement for a post-ligation cleanup step before electroporation. A 15-minute heat denaturation at 70°C is all that is needed prior to cell transformation.
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| Figure 1. Ligation efficiencies measured by transformation. Ligation reactions were heat denatured at 70°C and used without additional cleanup to transform electrocompetent E. cloni® 10G cells. Transformants (in millions) are shown. |
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The CloneDirect Rapid Ligation Kit contains: 10X CloneDirect Buffer (contains ATP), CloneSmart® DNA Ligase, and complete protocols.
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