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- Lucigen
- http://lucigen.com/store/docs/manuals/MA120-T4-Lig-HC.pdf
- 30243-2
- 2026年01月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20º C
- 英文名：
NxGen® T4 DNA Ligase (High Concentration)
- 库存：
大量
- 供应商：
中北林格
- 规格：
7,500 U
NxGen® T4 DNA Ligase
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Reliably join double-stranded nucleic acid chains.
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
| T4 DNA Ligase Version | Sizes | Ligase Concentration | Provided Buffer |
| Low Concentration Ligase | 1,500 U |
2 U/µL | 10X T4 DNA Ligase Buffer |
| High Concentration Rapid Kit | 1,500 U 7,500 U |
10 U/µL | 2X Rapid Ligation Buffer, 10X T4 DNA Ligase Buffer |
10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl2, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.
2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mM MgCl2, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.
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Figure 1: Purity |
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Figure 2: Exonuclease and Endonuclease Activity Assay |
Unit Definition: One Weiss Unit is defined as the amount of enzyme required to convert 1 nmol of 32P-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37 °C, using specified reaction conditions. Note: 1 Weiss Unit is approximately 67 cohesive end units.
Source: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.
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Ordering
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Specifications
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Manuals
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Resources
Storage buffer: T4 DNA Ligase is supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 0.1%Triton X-100, 50% glycerol, pH 7.5 @ 25 °C.
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文献和实验Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
Joining RNA Molecules with T4 DNA Ligase
Researchers interested in studying RNA structure and function or RNA-protein interactions are increasingly using site-specifically modified RNAs to probe sites of interest (1 ). In addition to expanding the repertoire of functional groups
Splint Ligation of RNA with T4 DNA Ligase
Splint ligation of RNA, whereby specific RNA molecules are ligated together, can be carried out using T4 DNA ligase and a bridging DNA oligonucleotide complementary to the RNAs. This method takes advantage of the property of T4 DNA ligase
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