- ￥1547.91
- Lucigen
- http://lucigen.com/store/docs/manuals/MA115-M-MuLV.pdf
- 30222-1
- 2026年06月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20º C
- 英文名：
NxGen® M-MuLV Reverse Transcriptase
- 库存：
大量
- 供应商：
中北林格
- 规格：
50,000 U
NxGen® M-MuLV Reverse Transcriptase
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Outstanding cDNA synthesis.
M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.
Specifications
Unit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
Source: A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.
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| Unit Concentration | 200,000 U/mL |
| Purity (SDS-PAGE) | >99% |
| SS Exonuclease | 2,000 U <5.0% released |
| DS Exonuclease | 2,000 U <0.5% released |
| Endonuclease | 2,000 U <10% converted |
| E. coli 16S rDNA Contamination | 2,000 U <10 copies |
| Storage | -20°C |
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Ordering
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Manuals
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Resources
Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25°C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25°C. 250,000 U is supplied as 5 × 50,000 U.
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文献和实验3. a) Add RNA to beads. b) Heat to 70 C for 2 min and cool slowly to RT for 10 min. c) Bind beads. d) Remove liquid. 4. Resuspend beads in : 2.5 ul Buffer A* (200 mM Tris-HCl, pH 8.3,1.0 M KCl) 2.5 ul Buffer B* (30 mM MgCl2 and 15 mM
in : 2.5 ul Buffer A* (200 mM Tris -HCl, pH 8.3,1.0 M KCl) 2.5 ul Buffer B* (30 mM MgCl2 and 15 mM MnSO4) 20.0 ul dNTPs (2.5 mM each) 1.0 ul 32P-dCTP (5 uCi) 1.0 ul RNasin-Pharmacia 2.0 ul
b) Heat to 70 C for 2 min and cool slowly to RT for 10 min. c) Bind beads. d) Remove liquid. 4. Resuspend beads in : 2.5 ul Buffer A* (200 mM Tris-HCl, pH 8.3,1.0 M KCl) 2.5 ul Buffer B* (30 mM MgCl2 and 15 mM MnSO4) 20.0 ul dNTPs
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