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- http://lucigen.com/store/docs/manuals/MA077-10-mM-dNTP.pdf
- 30029-1
- 2026年01月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20º C
- 英文名：
10 mM dNTP Set, PCR Grade,
- 库存：
大量
- 供应商：
中北林格
- 规格：
750 uL each dNTP
PCR Grade dNTPs
- Convenient formulations.
- Highest quality: > 99% purity.
- Tested in PCR.
- Great value!
Highest quality deoxynucleotides, 2'-deoxynucleoside 5'-triphosphates, at a great value, formulated for your convenience.
These nucleotides are also suitable for:
- sequencing
- nick translation
- cDNA synthesis
- TdT-tailing reactions
- fill-in reactions
2.5 mM dNTP Mix, PCR Grade
This ready-to-use dNTP Mix is a mixture of all four nucleotides (dATP, dCTP, dGTP, dTTP), each dissolved in highly purified water at a concentration of 2.5 mM, at pH 8.3. Two milliliters of the Mix is enough for 500 standard 50 µlEconoTaq® and TaqSelect™ DNA Polymerase PCR reactions. Free sample available.
10 mM dNTP Set, PCR Grade
This high-purity dNTP set allows you to formulate your own PCR mix. Each of all four nucleotides (dATP, dCTP, dGTP, dTTP), is provided in a separate vial (750 µl each), dissolved at a concentration of 10 mM in highly purified water, pH 8.3.
PLEASE NOTE
Some applications in which Lucigen’s dNTPs can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used. The PCR process is the subject of European Patent Nos. 201,184 and 200,262 owned by Hoffman-LaRoche. Those patents expired on March 28, 2006. The corresponding PCR process patents in the United States expired on March 29, 2005. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties.
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文献和实验Troubleshooting for PCR and multiplex PCR
some/all of the above 10. Longer PCR products in my multiplex reaction are weak. How can I improve their yield? Decrease KCl (buffer) concentration to 0.7-0.8x, but keep MgCl2 concentration at 1.5-2mM Increase MgCl2 concentration up to 3-4.5 mM but keep dNTP
过的 DNA 聚合酶增强了与模板的亲和度，其灵敏度更高，因而所需的模板量少。过低的模板量会减少 PCR 的产量，过高的模板量可能造成非特异性扩增，所以 DNA 模板量的优化尤为重要。 除了 gDNA、cDNA 和质粒 DNA，PCR 产物也可作为模板以获得更高的产量。但 PCR 产物中存在的残余反应组分（如引物、dNTP、盐和副产物）可能会影响下一次的 PCR 反应。所以推荐在下一次 PCR 前使用 PCR 产物纯化试剂盒将 PCR 产物先进行纯化。 【DNA 聚合酶】 DNA 聚合酶是PCR扩增中最关键
Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR
HCl. 5. Vortex. The DNA is now in a 20 mM Tris/0.1 mM EDTA at pH 8.1 with about 12.5 mM Na++ ions. That's pretty close to the 10 mM Tris/ 0.1 mM EDTA / pH 8 solution used to store DNA. 6. Use 1 µl for PCR 7. (May need to microfuge for 6 minutes
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