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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Bone sialoprotein 2 (IBSP), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Bone sialoprotein 2 (IBSP), partial品名简称:
Recombinant Human IBSP protein, partial货号:
CSB-EP010945HU(A4)规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
IBSPUniprot No.:
P21815别名:
Bone sialoprotein II种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
32.4 kDa表达区域:
129-281aa氨基酸序列:
AIQLPKKAGDITNKATKEKESDEEEEEEEEGNENEESEAEVDENEQGINGTSTNSTEAENGNGSSGGDNGEEGEEESVTGANAEDTTETGRQGKGTSKTTTSPNGGFEPTTPPQVYRTTSPPFGKTTTVEYEGEYEYTGANEYDNGYEIYESE蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.功能2:
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.文献:
"Human bone sialoprotein. Deduced protein sequence and chromosomal localization." Fisher L.W., McBride O.W., Termine J.D., Young M.F. J. Biol. Chem. 265:2347-2351(1990)研究领域:
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文献和实验Detection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone Sections
, including osteocalcin, osteopontin, osteonectin, bone sialoprotein, decorin, and biglycan. Of this group only osteocalcin and bone sialoprotein are specific for bone, whereas the other proteins are also present in other, noncalcifying, tissues. The knowledge
不是处在同一位置. (5)免疫金电镜分析进行蛋白质定位研究,例如青蛙卵母细胞核酸包膜中端粒结合蛋白质的定位研究. (6)BSP(Bone sialoprotein)和OPN(osteopontin)是胶原矿化组织中两种主要的非胶状蛋白质,可利用胶体金对细胞进行包埋处理,来跟踪上述物质,来分析其在生命活动中的作用. (7)胶体金标记、高分辨扫描电子显微镜研究疱疹病毒和被感染细胞表面的病毒单一糖蛋白,发现用非包层的方法来标记的观察效果要优于传统的包层方法.
,vonKossa染色形成黑色的结节。BMSC经成骨诱导后7天即可表现出碱性磷酸酶的显著增加。各种成骨特异性蛋白,如骨钙蛋白(osteocalcin,OCN)、骨桥蛋白(osteopontin,0PN)以及骨涎蛋白(bone sialoprotein,BSP)等,也陆续开始表达。 3.BMSC修复关节软骨缺损软骨缺损是创伤治疗领域的一大难题。由外伤、肿瘤或软骨退行性病变所引起的关节软骨缺损,在临床上尚无有效的修复方法,最终将导致关节功能丧失,甚至残废,给患者带来严重的身心痛苦。人工关节置换术目前虽颇为盛行
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