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- 2025年07月15日
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文献和实验Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
Materials Mouse with induced lesion (Basic Protocol protocol 22 or protocol 33 ) Tissue‐Tek (Sakura
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
are the Worthington product Codes: TL, TRL, TRLS, and TRLVMF. These products are suitable for cell harvesting as well as tissue dissociation. Elastase Pancreatic elastase is a serine protease with a specificity for peptide bonds adjacent
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