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文献和实验Transgenic Mouse and Transgenic Rat Outline
such as lacZ or a transcription factor). DNA is prepared and microinjected into fertilized mouse eggs. Potentially transgenic mice or rats are born. Transgenic founders are identified, usually by a simple PCR assay, and bred to produce offspring for analysis
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
CalbiochemReferenceWeskamp, G., and Otten, U. (1987). An enzyme-linked immunoassay for nerve growth factor (NGF): A tool for studying regulatory mechanisms involved in ngf production in brain and in peripheral tissues. Journal of Neurochemistry, 48 (6),1779-1786.
Models of Inflammation: Carrageenan Air Pouch
) ELISA kit(s) specific for inflammatory mediators (e.g., TNF‐α, IL‐1β, IL‐6, IL‐10, prostaglandins; Thermo Scientific) Isotonic phosphate‐buffered saline (PBS
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