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文献和实验Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells
are then differentiated along the endothelial lineage using endothelial growth medium supplemented with 40 ng/mL vascular endothelial growth factor (VEGF). The differentiated endothelial population expresses both Fetal Liver Kinase 1 (Flk-1) and VE-Cadherin on the cell
In Vitro Derivation and Expansion of Endothelial Cells From Embryonic Stem Cells
ES cells using Flk-1-positive cells, vascular endothelial growth factor supplementation, and a highly selective manual selection technique. This methodology, although rigorous, overcomes two current obstacles in ES derivation and culture by generating
TGF- Signaling in Embryonic Stem Cell-Derived Endothelial Cells
The roles of the transforming growth factor (TGF)-β superfamily in vasculogenesis have been implicated by the findings that mutations in genes encoding for various TGF-β superfamily signaling components exhibit defects in vascular tissues
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