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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
. For cytospin slides : i) fix air dried cell samples with a freshly prepared paraformaldeyde solution (4% in PBS, pH 7.4) for 30 min at room temperature; ii) rinse slides with PBS and incubate in permeabilisation solution (0.1% Triton X-100, 0.1% sodium citrate
Scanning the human genome with combinatorial transcription factor libraries Published online: 18 February 2003, doi:10.1038/nbt794 March 2003 Volume 21 Number 3 pp 269 - 274 Pilar Blancafort, Laurent Magnenat & Carlos F
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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