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- 2025年07月15日
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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
., Koesling, D., and Schneider‐Mergener, J. 1999. Interaction of a PDZ protein domain with a synthetic library of all human protein C termini. Angew. Chem. Int. Ed. 38:2000‐2004.
Strategies to Optimize Protein Expression in E. coli
characterization of the spine‐associated RapGAP (SPAR) PDZ domain. Protein Expr. Purif. 62:9‐14. Brown, B.L., Grigoriu, S., Kim, Y., Arruda, J.M., Davenport, A., Wood, T.K., Peti, W
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